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Literature summary for 2.7.9.2 extracted from
- Phosphoenolpyruvate synthetase from the hyperthermophilic archaeon Pyrococcus furiosus (2001), J. Bacteriol., 183, 709-715.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| additional information | not affected by Na+ | Pyrococcus furiosus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | kinetics | Pyrococcus furiosus | |
| 0.11 | - |
pyruvate | pH 8.4, 80°C | Pyrococcus furiosus |  |
| 0.39 | - |
ATP | pH 8.4, 80°C | Pyrococcus furiosus | |
| 0.4 | - |
phosphoenolpyruvate | pH 8.4, 80°C | Pyrococcus furiosus | |
| 1 | - |
AMP | pH 8.4, 80°C | Pyrococcus furiosus | |
| 38.4 | - |
phosphate | pH 8.4, 80°C | Pyrococcus furiosus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytoplasm | 5% of total cytoplasmic protein | Pyrococcus furiosus | 5737 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | enzyme contains 1 Ca atom per subunit | Pyrococcus furiosus | |
| Co2+ | requires divalent cations in the forward reaction, 93% of the activity with Mg2+ | Pyrococcus furiosus | |
| Iron | 0.1-0.4 g-atoms per subunit, no functional part of the enzyme | Pyrococcus furiosus | |
| K+ | 50% increased activity at 100 mM | Pyrococcus furiosus | |
| Mg2+ | slightly inhibitory above 10 mM | Pyrococcus furiosus | |
| Mg2+ | requires divalent cations in the forward reaction, Mg2+ is most effective | Pyrococcus furiosus | |
| Mn2+ | requires divalent cations in the forward reaction, 95% of the activity with Mg2+ | Pyrococcus furiosus | |
| additional information | Ca2+ and Sr2+ cannot substitute for Mg2+ in the catalytic reaction | Pyrococcus furiosus | |
| additional information | not affected by Na+ | Pyrococcus furiosus | |
| NH4+ | 48% increased activity at 100 mM | Pyrococcus furiosus | |
| Ni2+ | requires divalent cations in the forward reaction, 15% of the activity with Mg2+ | Pyrococcus furiosus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 92000 | - |
8 * 92000, SDS-PAGE, 30% of the enzyme is purified as a large inactive complex of about 1640 kDa | Pyrococcus furiosus |
| 690000 | - |
gel filtration | Pyrococcus furiosus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + pyruvate + H2O | Pyrococcus furiosus | involved in gluconeogenesis | AMP + phosphoenolpyruvate + phosphate | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pyrococcus furiosus | - |
hyperthermophilic archaeon | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| 19.4fold | Pyrococcus furiosus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 14.9 | - |
purified enzyme | Pyrococcus furiosus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + 2-ketobutyrate + H2O | 8% of the activity with pyruvate | Pyrococcus furiosus | ? | - |
? | |
| ATP + 2-oxoglutarate + H2O | 2% of the activity with pyruvate | Pyrococcus furiosus | ? | - |
? | |
| ATP + pyruvate + H2O | absolutely specific for ATP, highly specific for pyruvate | Pyrococcus furiosus | AMP + phosphoenolpyruvate + phosphate | - |
r | |
| ATP + pyruvate + H2O | phosphorylated enzyme as an intermediate | Pyrococcus furiosus | AMP + phosphoenolpyruvate + phosphate | - |
r | |
| ATP + pyruvate + H2O | equilibrium lies far to the side of phosphoenolpyruvate formation | Pyrococcus furiosus | AMP + phosphoenolpyruvate + phosphate | - |
r | |
| ATP + pyruvate + H2O | involved in gluconeogenesis | Pyrococcus furiosus | AMP + phosphoenolpyruvate + phosphate | - |
r | |
| additional information | no activity with 2-keto-3-methylvalerate, 2-ketoisocaproate, 2-ketomalonate, phenylpyruvate, hydroxyphenylpyruvate, imidazolpyruvate, and indole-3-pyruvate | Pyrococcus furiosus | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| octamer | 8 * 92000, SDS-PAGE, 30% of the enzyme is purified as a large inactive complex of about 1640 kDa | Pyrococcus furiosus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| PpsA | - |
Pyrococcus furiosus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 90 | - |
at pH 8.4 | Pyrococcus furiosus |
| 90 | - |
phosphoenolpyruvate formation | Pyrococcus furiosus |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 90 | optimum at 90°C, 0.2% of the optimal activity at 30°C | Pyrococcus furiosus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 90 | - |
t1/2: 9 h | Pyrococcus furiosus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 8.7 | - |
AMP | pH 8.4, 80°C | Pyrococcus furiosus | |
| 11.9 | - |
phosphate | pH 8.4, 80°C | Pyrococcus furiosus | |
| 12.6 | - |
phosphoenolpyruvate | pH 8.4, 80°C | Pyrococcus furiosus | |
| 1330 | - |
ATP | pH 8.4, 80°C | Pyrococcus furiosus | |
| 1570 | - |
pyruvate | pH 8.4, 80°C | Pyrococcus furiosus | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
at 80°C | Pyrococcus furiosus |
| 7.5 | - |
pyruvate formation | Pyrococcus furiosus |
| 9 | - |
at 80°C | Pyrococcus furiosus |
| 9 | - |
phosphoenolpyruvate formation | Pyrococcus furiosus |
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