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Literature summary for 2.7.9.3 extracted from
- Crystallization and preliminary X-ray diffraction analysis of selenophosphate synthetases from Trypanosoma brucei and Leishmania major (2013), Acta Crystallogr. Sect. F, 69, 864-867.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| recombinant expression of His6-tagged enzyme with a thrombin-cleavage site in Escherichia coli strain BL21(DE3) | Leishmania major |
| recombinant expression of His6-tagged enzyme with a thrombin-cleavage site in Escherichia coli strain BL21(DE3) | Trypanosoma brucei brucei |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| recombinant His6-tagged enzyme, from 0.1 M trisodium citrate, pH 5.6, 17% w/v, PEG 3350 using the microbatch method, 2 months, X-ray diffraction structure determination and analysis at 3.40 A resolution | Trypanosoma brucei brucei |
| the recombinant full-length enzyme cannot be crystallized, but the DELTAN-LmSPS2, which lacks residues 1-69, is efficiently crystallized, from 0.2 M ammonium sulfate, 0.1 M trisodium citrate, pH 5.2, and 21% w/v PEG 4000, 3 days, X-ray diffraction structure determination and analysis at 1.88 A resolution | Leishmania major |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | construction of an N-terminal truncation mtant enzyme lacking the first 69 amino acids, DELTAN-LmSPS2 | Leishmania major |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | required | Leishmania major |  |
| Mg2+ | required | Trypanosoma brucei brucei | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 44700 | - |
- |
Leishmania major |
| 45000 | - |
- |
Trypanosoma brucei brucei |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + selenide + H2O | Leishmania major | - |
AMP + selenophosphate + phosphate | - |
? | |
| ATP + selenide + H2O | Trypanosoma brucei brucei | - |
AMP + selenophosphate + phosphate | - |
? | |
| ATP + selenide + H2O | Trypanosoma brucei brucei 927 / 4 GUTat10.1 / TREU927 | - |
AMP + selenophosphate + phosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Leishmania major | Q4Q0M0 | - |
- |
| Trypanosoma brucei brucei | Q38A34 | - |
- |
| Trypanosoma brucei brucei 927 / 4 GUTat10.1 / TREU927 | Q38A34 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, and gel filtration | Leishmania major |
| recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, and gel filtration | Trypanosoma brucei brucei |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + selenide + H2O | - |
Leishmania major | AMP + selenophosphate + phosphate | - |
? | |
| ATP + selenide + H2O | - |
Trypanosoma brucei brucei | AMP + selenophosphate + phosphate | - |
? | |
| ATP + selenide + H2O | - |
Trypanosoma brucei brucei 927 / 4 GUTat10.1 / TREU927 | AMP + selenophosphate + phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 44700, recombinant His6-tagged enzyme, SDS-PAGE | Leishmania major |
| ? | x * 45000, recombinant His6-tagged enzyme, SDS-PAGE | Trypanosoma brucei brucei |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| LmSPS2 | - |
Leishmania major |
| selenophosphate synthetase | - |
Leishmania major |
| selenophosphate synthetase | - |
Trypanosoma brucei brucei |
| SPS | - |
Leishmania major |
| SPS | - |
Trypanosoma brucei brucei |
| TbSPS2 | - |
Trypanosoma brucei brucei |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | - |
Leishmania major | |
| ATP | - |
Trypanosoma brucei brucei | |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Leishmania major | isoelectric focusing, recombinant His6-tagged enzyme | - |
5.83 |
| Trypanosoma brucei brucei | isoelectric focusing, recombinant His6-tagged enzyme | - |
5.98 |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | the enzyme plays an indispensable role in selenium metabolism, being responsible for catalyzing the activation of selenide with adenosine 5'-triphosphate (ATP) to generate selenophosphate, the essential selenium donor for selenocysteine synthesis | Trypanosoma brucei brucei |
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