Literature summary for 2.7.9.3 extracted from

Tobe, R.; Carlson, B.A.; Huh, J.H.; Castro, N.P.; Xu, X.M.; Tsuji, P.A.; Lee, S.G.; Bang, J.; Na, J.W.; Kong, Y.Y.; Beaglehole, D.; Southon, E.; Seifried, H.; Tessarollo, L.; Salomon, D.S.; Schweizer, U.; Gladyshev, V.N.; Hatfield, D.L.; Lee, B.J.
Selenophosphate synthetase 1 is an essential protein with roles in regulation of redox homoeostasis in mammals (2016), Biochem. J., 473, 2141-2154.

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Cloned(Commentary)

Cloned (Comment)	Organism
gene Sps1, located on chromosome 2, expression anaysis	Mus musculus

Protein Variants

Protein Variants	Comment	Organism
additional information	generation of a Sps1 knockout mouse with systemic isozyme SPS1 deficiency via C57BL/6 x 129/SvJae embryonic stem cell electroporation. Embryonic lethality of the Sps1-/- mice. Targeted removal of SPS1 in F9 cells, a mouse embryonal carcinoma cell line, phenotype, overview. Construction of a knockdown-resistant (rescue) SPS1 expression vector via three silent point mutations that are introduced into the shRNA target sequence by two-step PCR	Mus musculus

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	required	Mus musculus

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
ATP + selenide + H2O	Mus musculus	-	AMP + selenophosphate + phosphate	-	?
ATP + selenide + H2O	Mus musculus C57BL/6	-	AMP + selenophosphate + phosphate	-	?

Organism

Organism	UniProt	Comment	Textmining
Mus musculus	Q8BH69	gene SPS1	-
Mus musculus C57BL/6	Q8BH69	gene SPS1	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
embryo	-	Mus musculus	-
F-9 cell	-	Mus musculus	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + selenide + H2O	-	Mus musculus	AMP + selenophosphate + phosphate	-	?
ATP + selenide + H2O	-	Mus musculus C57BL/6	AMP + selenophosphate + phosphate	-	?

Synonyms

Synonyms	Comment	Organism
selenophosphate synthetase 1	-	Mus musculus
SPS	-	Mus musculus
SPS1	-	Mus musculus

Cofactor

Cofactor	Comment	Organism	Structure
ATP	-	Mus musculus

General Information

General Information	Comment	Organism
malfunction	systemic SPS1 deficiency in Sps1 knockout mice leads to embryos are clearly underdeveloped by E8.5 and virtually resorbed by E14.5. The knockout of Sps1 in the liver preserves viability, but significantly affects the expression of a large number of mRNAs involved in cancer, embryonic development, and the glutathione system, especially extreme deficiency of glutaredoxin 1 (GLRX1) and glutathione-S-transferase omega 1. Targeted removal of SPS1 in F9 cells, a mouse embryonal carcinoma cell line, affects the glutathione system proteins and accordingly leads to the accumulation of hydrogen peroxide in the cell. Hydrogen peroxide accumulates due to the downregulation of GLRX1 in SPS1-deficient F9 cells. Overexpression of mouse or human GLRX1 leads to a reversal of observed increases in reactive oxygen species in the F9 SPS1/GLRX1-deficient cells and result in levels that are similar to those in F9 SPS1-sufficient cells. Loss of Sps1 in the liver affects iron and manganese levels. The expression of genes encoding proteins responsible for the de novo synthesis of glutathione, such as glutamate cysteine ligase catalytic subunit (GCLC), glutamate-cysteine modifier subunit (GCLM), and glutathione synthetase (GSS), is not affected by a deficiency in SPS1	Mus musculus
physiological function	isozyme SPS1 is an essential mammalian enzyme with roles in regulating redox homeostasis and controlling cell growth. Isozyme SPS1 plays a role in supporting and/or sustaining cancer	Mus musculus

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Release 2023.1 (January 2023)