Cloned (Comment) | Organism |
---|---|
in pKGWFS7 for expression N-terminally of an (eGFP)-beta-glucoronidase fusion and Agrobacterium-mediated transformation of Arabidopsis plants, in pK7FWG2 (as full-length or 158 N-terminal amino acid fragment) for expression as eGFP-fusion and subcellular localization in onion epidermis and Arabidopsis thaliana, in pPS48uYFP as full-length, C-terminal truncations (N-terminal 610, 158, or 61 amino acids) or C-terminally fused to transit peptide of AtGWD isoform 1 for Agrobacterium-mediated expression in Nicotiana benthamiana, in pGEM-4Z (as full-length or as fusion with transit peptide of AtGWD1) for in vitro transcription, translation and chloroplast import assays | Arabidopsis thaliana |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cell wall | along the cell wall, N-terminal 158 and 61 amino acids (revealed by confocal laser scanning microscopy) | Arabidopsis thaliana | 5618 | - |
additional information | extra-plastidial localisation of full-length enzyme (in vitro chloroplast import assay), lack of chloroplast transit peptide | Arabidopsis thaliana | - |
- |
additional information | no fluorescence signal detected for full-length enzyme or its 610 N-terminal residues fused to eYFP or eGFP | Arabidopsis thaliana | - |
- |
nucleus | N-terminal 158 and 61 amino acids (revealed by confocal laser scanning microscopy) | Arabidopsis thaliana | 5634 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + alpha-glucan + H2O | Arabidopsis thaliana | exclusive phosphorylation of C-6 position, in vitro | AMP + phospho-alpha-glucan + phosphate | - |
? | |
additional information | Arabidopsis thaliana | not involved in transient starch degradation, plants lacking AtGWD show similar starch and sugar content as wild-type plants | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | Q9STV0 | ecotype Columbia-0, wild-type or mutant lacking AtGWD2 | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
phosphoprotein | autophosphorylation | Arabidopsis thaliana |
Purification (Comment) | Organism |
---|---|
by metal-affinity chromatography after expression in Saccharomyces cerevisiae with C-terminal V5- and hexa-His-tag | Arabidopsis thaliana |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
abscission zone | floral organ, strong expression in vascular tissue just before onset of senescence (promoter activity) | Arabidopsis thaliana | - |
cauline leaf | high expression level (microarray) | Arabidopsis thaliana | - |
companion cell | phloem, high expression just before onset of senescence (promoter activity) | Arabidopsis thaliana | - |
flower | strong expression in mature flower parts (microarray) | Arabidopsis thaliana | - |
leaf | strong expression in vascular tissue (abaxial side of primary vascular bundle, phloem) just before onset of senescence (promoter activity), senescent leaves (microarray) | Arabidopsis thaliana | - |
additional information | spatially (vascular) and temporarily (age-dependent) restricted expression pattern (promoter activity) | Arabidopsis thaliana | - |
root | strong expression in vascular tissue just before onset of senescence (promoter activity) | Arabidopsis thaliana | - |
sepal | strong expression in vascular tissue just before onset of senescence (promoter activity) | Arabidopsis thaliana | - |
silique | strong expression in vascular tissue (floral organ abscission zone, dehiscence zone between silique walls, vascular tissue of silique walls) just before onset of senescence (promoter activity, microarray) | Arabidopsis thaliana | - |
stamen | strong expression in vascular tissue just before onset of senescence (promoter activity) | Arabidopsis thaliana | - |
stem | strong expression in vascular tissue just before onset of senescence (promoter activity) | Arabidopsis thaliana | - |
vascular tissue | leaf, stem, root, flower, silique only just before onset of senescence (promoter activity) | Arabidopsis thaliana | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.02 | - |
amylopectin, mean chain length: 23.5, nmol glucose-6-phosphate/mg: 0.1 | Arabidopsis thaliana |
0.04 | - |
amylopectin, pre-phosphorylated, mean chain length: 24.8, nmol glucose-6-phosphate/mg: 0.5 | Arabidopsis thaliana |
0.49 | - |
amylopectin, elongated, mean chain length: 29.7, nmol glucose-6-phosphate/mg: 0.1 | Arabidopsis thaliana |
0.65 | - |
amylopectin, elongated and pre-phosphorylated, mean chain length: 30.8, nmol glucose-6-phosphate/mg: 39.4 | Arabidopsis thaliana |
1.36 | - |
soluble potato starch | Arabidopsis thaliana |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + alpha-glucan + H2O | exclusive phosphorylation of C-6 position, in vitro | Arabidopsis thaliana | AMP + phospho-alpha-glucan + phosphate | - |
? | |
ATP + amylopectin + H2O | 1 h, 30°C, pH 7, in presence of 10 mM NH4Cl, 10 mM MgCl2, 0.5 mM dithiothreitol, 0.2 mg/ml bovine serum albumin, substrates: waxy maize amylopectin (unmodified (glycogen), elongated by phosphorylase a, pre-phosphorylated by potato glucan, water dikinase (GWD), or elongated and pre-phosphorylated) or soluble potato starch | Arabidopsis thaliana | AMP + phospho-amylopectin + phosphate | reaction stop by boiling | ? | |
additional information | not involved in transient starch degradation, plants lacking AtGWD show similar starch and sugar content as wild-type plants | Arabidopsis thaliana | ? | - |
? | |
additional information | activity is independent of pre-phosphorylation of glucan substrate | Arabidopsis thaliana | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
alpha-glucan, water dikinase | - |
Arabidopsis thaliana |
AtGWD2 | At4g24450 | Arabidopsis thaliana |