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Literature summary for 2.7.9.4 extracted from
- Light-induced degradation of storage starch in turions of Spirodela polyrhiza depends on nitrate (2008), Plant Cell Environ., 31, 1460-1469.
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 154000 | - |
starch-associated, phosphorylated and non-phosphorylated GWD, SDS-PAGE | Spirodela polyrhiza |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + alpha-glucan + H2O | Spirodela polyrhiza | light-induced GWD phosphorylation and subsequent starch breakdown only in presence of sufficient amount of nitrogen to ensure utilisation of released carbohydrate | AMP + phospho-alpha-glucan + phosphate | - |
? |  |
| ATP + alpha-glucan + H2O | Spirodela polyrhiza SJ | light-induced GWD phosphorylation and subsequent starch breakdown only in presence of sufficient amount of nitrogen to ensure utilisation of released carbohydrate | AMP + phospho-alpha-glucan + phosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Spirodela polyrhiza | - |
Araceae | - |
| Spirodela polyrhiza SJ | - |
Araceae | - |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| phosphoprotein | autophosphorylation upon irradiation with continuous red light leads dependent on presence of nitrate | Spirodela polyrhiza |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| extracted from starch granules with SDS, washed with methanol and chloroform and identified by 2D-SDS-PAGE and immunodetection | Spirodela polyrhiza |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| turion | - |
Spirodela polyrhiza | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + alpha-glucan + H2O | light-induced GWD phosphorylation and subsequent starch breakdown only in presence of sufficient amount of nitrogen to ensure utilisation of released carbohydrate | Spirodela polyrhiza | AMP + phospho-alpha-glucan + phosphate | - |
? | |
| ATP + alpha-glucan + H2O | light-induced GWD phosphorylation and subsequent starch breakdown only in presence of sufficient amount of nitrogen to ensure utilisation of released carbohydrate | Spirodela polyrhiza SJ | AMP + phospho-alpha-glucan + phosphate | - |
? | |
| starch + ATP + H2O | starch phosphorylation upon irradiation of dormant turions (1-2 days, 25°C) dependent on presence of nitrate (8 mM KNO3 and 1 mM Ca(NO3)2) or 10 mM NH4Cl (as nitrogen source) in nutrient medium | Spirodela polyrhiza | AMP + phospho-starch + phosphate | - |
? | |
| starch + ATP + H2O | starch phosphorylation upon irradiation of dormant turions (1-2 days, 25°C) dependent on presence of nitrate (8 mM KNO3 and 1 mM Ca(NO3)2) or 10 mM NH4Cl (as nitrogen source) in nutrient medium | Spirodela polyrhiza SJ | AMP + phospho-starch + phosphate | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| glucan, water dikinase | starch-associated | Spirodela polyrhiza |
| GWD | - |
Spirodela polyrhiza |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Spirodela polyrhiza | phosphorylated GWD | - |
4 |
| Spirodela polyrhiza | non-phosphorylated GWD | - |
5.5 |
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Release 2023.1 (January 2023)
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