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Literature summary for 2.8.1.1 extracted from
- Purification and some properties of Trametes sanguinea rhodanese (1973), Agric. Biol. Chem., 37, 629-635.
No PubMed abstract available
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2-mercaptoethanol | at pH 8.0 | Trametes sanguinea |  |
| CN- | substrate inhibition at high concentrations | Trametes sanguinea | |
| iodoacetate | - |
Trametes sanguinea | |
| L-cysteine | at pH 8.0 | Trametes sanguinea | |
| p-chloromercuribenzoate | at pH 9.5, not at pH 8.0 | Trametes sanguinea | |
| sodium arsenite | - |
Trametes sanguinea | |
| Sodium borohydride | pH 8.0 | Trametes sanguinea | |
| thiosulfate | substrate inhibition at high concentrations | Trametes sanguinea | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 8.3 | - |
thiosulfate | 25°C, pH 8.5 | Trametes sanguinea | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 51700 | - |
gel filtration | Trametes sanguinea |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| thiosulfate + cyanide | Trametes sanguinea | - |
sulfite + thiocyanate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Trametes sanguinea | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Trametes sanguinea |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| culture filtrate | - |
Trametes sanguinea | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 15.9 | - |
Sephadex G-100 | Trametes sanguinea |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| thiosulfate + benzene thiosulfonate | - |
Trametes sanguinea | ? | - |
? | |
| thiosulfate + cyanide | - |
Trametes sanguinea | sulfite + thiocyanate | - |
? | |
| thiosulfate + dihydrolipoate | - |
Trametes sanguinea | ? | - |
? | |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | - |
10 min, pH 5.0, stable up to | Trametes sanguinea |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | 8.5 | - |
Trametes sanguinea |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 11 | pH 6.0: about 75% of activity maximum, pH 11.0: about 70% of activity maximum | Trametes sanguinea |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 8 | 30°C, 44 h, stable | Trametes sanguinea |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Trametes sanguinea | - |
- |
5.1 |
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