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Literature summary for 2.8.1.1 extracted from

  • Miller-Martin, D.M.; Chirgwin, J.M.; Horowitz, P.M.
    Mutations of noncatalytic sulfhydryl groups influence the stability, folding, and oxidative susceptibility of rhodanese (1994), J. Biol. Chem., 269, 3423-3428.
    View publication on PubMed
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Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Bos taurus

Protein Variants

Protein Variants Comment Organism
C247S no activity to thiosulfate Bos taurus
C254S more resistant than wild-type to inactivation by dithiothreitol, more readily reactivated following oxidative inactivation and increased exposure of hydrophobic surfaces following removal of the transferable sulfur Bos taurus
C254S/C263S less stable in urea than wild type, more resistant than wild-type to inactivation by dithiothreitol, more readily reactivated following oxidative inactivation and increased exposure of hydrophobic surfaces following removal of the transferable sulfur Bos taurus
C263S less stable in urea than wild type Bos taurus
C63S less stable in urea than wild type Bos taurus
C63S/C254S/C263S less stable in urea than wild type, more resistant than wild-type to inactivation by dithiothreitol, more readily reactivated following oxidative inactivation and increased exposure of hydrophobic surfaces following removal of the transferable sulfur Bos taurus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
thiosulfate + cyanide Bos taurus
-
 sulfite + thiocyanate
-
 ?

Organism

Organism UniProt Comment Textmining
Bos taurus
-
-
-

Renatured (Commentary)

Renatured (Comment) Organism
-
 Bos taurus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
thiosulfate + cyanide
-
 Bos taurus sulfite + thiocyanate
-
 ?