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Literature summary for 2.8.1.1 extracted from
- Chinese hamster rhodanese cDNA: activity of the expressed protein is not blocked by a C-terminal extension (1995), Protein Expr. Purif., 6, 693-699.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Oncorhynchus mykiss |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | - |
Bos taurus |
| additional information | last 5 amino acids replaced by 28 residues of unrelated sequence, no differences to kinetic constants of wild-type enzyme | Oncorhynchus mykiss |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 5.6 | - |
thiosulfate | mutant rhodanese | Oncorhynchus mykiss |  |
| 7.9 | - |
thiosulfate | recombinant rhodanese | Oncorhynchus mykiss | |
| 9.2 | - |
thiosulfate | recombinant rhodanese | Bos taurus | |
| 9.3 | - |
thiosulfate | liver rhodanese | Bos taurus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 33000 | - |
1 * 33000, SDS-PAGE | Oncorhynchus mykiss |
| 33000 | 34000 | hydrophobic column chromatography | Oncorhynchus mykiss |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| thiosulfate + cyanide | Bos taurus | - |
sulfite + thiocyanate | - |
? | |
| thiosulfate + cyanide | Oncorhynchus mykiss | - |
sulfite + thiocyanate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bos taurus | - |
- |
- |
| Oncorhynchus mykiss | - |
chinese hamster | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Oncorhynchus mykiss |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| ovary | - |
Oncorhynchus mykiss | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 491 | - |
C-terminally extended mutant enzyme | Oncorhynchus mykiss |
| 818 | - |
recombinant wild-type | Oncorhynchus mykiss |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| thiosulfate + cyanide | - |
Bos taurus | sulfite + thiocyanate | - |
? | |
| thiosulfate + cyanide | - |
Oncorhynchus mykiss | sulfite + thiocyanate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | - |
Bos taurus |
| monomer | 1 * 33000, SDS-PAGE | Oncorhynchus mykiss |
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Release 2023.1 (January 2023)
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