Any feedback?
Literature summary for 2.8.1.1 extracted from
- A new sulfurtransferase from the hyperthermophilic bacterium Aquifex aeolicus. Being single is not so simple when temperature gets high (2007), FEBS J., 274, 4572-4587.
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| homology modeling of structure. The potential active site is located between a central beta strand and an alpha helix. Residues R30, E34 are involved in substrate binding | Aquifex aeolicus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.05 | - |
Polysulfide | pH 9.0, 80°C | Aquifex aeolicus | |
| 2.1 | - |
cyanide | pH 9.0, 80°C | Aquifex aeolicus |  |
| 5.7 | - |
thiosulfate | pH 9.0, 80°C | Aquifex aeolicus | |
| 6.9 | - |
tetrathionate | pH 9.0, 80°C | Aquifex aeolicus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| periplasm | - |
Aquifex aeolicus | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 12800 | - |
1 * 12804, calculated, 1 * 12800, mass spectrometry, enzyme is in equilibrium between monomer, dimer and tetramer with shift to the tetrameric state in presence of substrate | Aquifex aeolicus |
| 12800 | - |
2 * 12804, calculated, 2 * 12800, mass spectrometry, enzyme is in equilibrium between monomer, dimer and tetramer with shift to the tetrameric state in presence of substrate | Aquifex aeolicus |
| 12800 | - |
4 * 12804, calculated, 4 * 12800, mass spectrometry, enzyme is in equilibrium between monomer, dimer and tetramer with shift to the tetrameric state in presence of substrate | Aquifex aeolicus |
| 12804 | - |
1 * 12804, calculated, 1 * 12800, mass spectrometry, enzyme is in equilibrium between monomer, dimer and tetramer with shift to the tetrameric state in presence of substrate | Aquifex aeolicus |
| 12804 | - |
2 * 12804, calculated, 2 * 12800, mass spectrometry, enzyme is in equilibrium between monomer, dimer and tetramer with shift to the tetrameric state in presence of substrate | Aquifex aeolicus |
| 12804 | - |
4 * 12804, calculated, 4 * 12800, mass spectrometry, enzyme is in equilibrium between monomer, dimer and tetramer with shift to the tetrameric state in presence of substrate | Aquifex aeolicus |
| 15000 | 50000 | - |
Aquifex aeolicus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aquifex aeolicus | - |
- |
- |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 850 | - |
pH 9.0, 80°C | Aquifex aeolicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| polysulfide + cyanide | - |
Aquifex aeolicus | ? + thiocyanate | - |
? | |
| tetrathionate + cyanide | - |
Aquifex aeolicus | ? + thiocyanate | - |
? | |
| thiosulfate + cyanide | - |
Aquifex aeolicus | sulfite + thiocyanate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 12804, calculated, 2 * 12800, mass spectrometry, enzyme is in equilibrium between monomer, dimer and tetramer with shift to the tetrameric state in presence of substrate | Aquifex aeolicus |
| monomer | 1 * 12804, calculated, 1 * 12800, mass spectrometry, enzyme is in equilibrium between monomer, dimer and tetramer with shift to the tetrameric state in presence of substrate | Aquifex aeolicus |
| tetramer | 4 * 12804, calculated, 4 * 12800, mass spectrometry, enzyme is in equilibrium between monomer, dimer and tetramer with shift to the tetrameric state in presence of substrate | Aquifex aeolicus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Aq-477 | - |
Aquifex aeolicus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 80 | - |
- |
Aquifex aeolicus |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
8% of maximum activity | Aquifex aeolicus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 9 | - |
- |
Aquifex aeolicus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
no residual activity below | Aquifex aeolicus |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
