Literature summary for 2.8.1.7 extracted from

Zheng, L.; White, R.H.; Cash, V.L.; Dean, D.R.
Mechanism for the desulfurization of L-cysteine catalyzed by the nifS gene product (1994), Biochemistry, 33, 4714-4720.

Search for more literature for 2.8.1.7

Protein Variants

Protein Variants	Comment	Organism
C325A	no cysteine desulfurase activity	Azotobacter vinelandii

Inhibitors

Inhibitors	Comment	Organism	Structure
L-Allylglycine	irreversible inactivation	Azotobacter vinelandii
Vinylglycine	irreversible inactivation	Azotobacter vinelandii

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.075	-	L-cysteine	-	Azotobacter vinelandii

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
L-cysteine + [enzyme]-cysteine	Azotobacter vinelandii	-	L-alanine + [enzyme]-S-sulfanylcysteine	-	?
additional information	Azotobacter vinelandii	enzyme participates in the biosynthesis of the nitrogenase metalloclusters by providing the inorganic sulfur required for Fe-S core formation	?	-	?

Organism

Organism	UniProt	Comment	Textmining
Azotobacter vinelandii	-	-	-

Reaction

Reaction	Comment	Organism	Reaction ID
L-cysteine + acceptor = L-alanine + S-sulfanyl-acceptor	mechanism	Azotobacter vinelandii	a

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
L-cysteine + [enzyme]-cysteine	-	Azotobacter vinelandii	L-alanine + [enzyme]-S-sulfanylcysteine	-	?
L-cysteine + [enzyme]-cysteine	intermediate is an enzyme-bound cysteinyl persulfide	Azotobacter vinelandii	L-alanine + [enzyme]-S-sulfanylcysteine	-	?
additional information	enzyme participates in the biosynthesis of the nitrogenase metalloclusters by providing the inorganic sulfur required for Fe-S core formation	Azotobacter vinelandii	?	-	?

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
additional information	-	activity is highly sensitive to temperature	Azotobacter vinelandii

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
additional information	-	activity is highly sensitive to pH	Azotobacter vinelandii

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
20	-	allylglycine	-	Azotobacter vinelandii

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Release 2023.1 (January 2023)