Literature summary for 2.8.1.7 extracted from

Lauhon, C.T.; Skovran, E.; Urbina, H.D.; Downs, D.M.; Vickery, L.E.
Substitutions in an active site loop of Escherichia coli IscS result in specific defects in Fe-S cluster and thionucleoside biosynthesis in vivo (2004), J. Biol. Chem., 279, 19551-19558.

Search for more literature for 2.8.1.7

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Escherichia coli

Protein Variants

Protein Variants	Comment	Organism
L333A	mutant defective in Fe-S biosynthesis in vivo but functional in persulfide formation and transfer in vitro	Escherichia coli
S326A	mutant defective in Fe-S biosynthesis in vivo but functional in persulfide formation and transfer in vitro	Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
L-cysteine + IscS	Escherichia coli	-	L-alanine + IscS-SSH	-	?
L-cysteine + IscS	Escherichia coli MC1061	-	L-alanine + IscS-SSH	-	?

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	-	-	-
Escherichia coli MC1061	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
purified as the N-terminal His6 fusion protein	Escherichia coli

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
0.0022	-	mutant S326A for s4U synthesis	Escherichia coli
0.0034	-	mutant S328A for s4U synthesis	Escherichia coli
0.0036	-	wild type for s4U synthesis	Escherichia coli
0.0038	-	mutant S336A for s4U synthesis	Escherichia coli
0.0043	-	mutant L333A for s4U synthesis	Escherichia coli

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
L-cysteine + IscS	-	Escherichia coli	L-alanine + IscS-SSH	-	?
L-cysteine + IscS	-	Escherichia coli MC1061	L-alanine + IscS-SSH	-	?

Synonyms

Synonyms	Comment	Organism
IscS	-	Escherichia coli

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Release 2023.1 (January 2023)