Literature summary for 2.8.1.7 extracted from

WU, A.; ZHANG, Y.; ZHENG, C.; DAI, Y.; LIU, Y.; ZENG, J.; GU, G.; LIU, J.
Purification and enzymatic characteristics of cysteine desulfurase, IscS, in Acidithiobacillus ferrooxidans ATCC 23270 (2008), Trans. Nonferrous Met. Soc. China, 18, 1450-1457.

No PubMed abstract available

Search for more literature for 2.8.1.7

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21(DE3) cells	Acidithiobacillus ferrooxidans

Inhibitors

Inhibitors	Comment	Organism	Structure
N-iodoacetyl-N'-(5-sulfo-1-naphthyl)ethylenediamine	up to 90% inhibition at 5 mM	Acidithiobacillus ferrooxidans

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.11	-	L-cysteine	in 50 mM Tris-HCl (pH 8.0), 10 mM MgCl2, 5 mM dithiothreitol, at 25°C	Acidithiobacillus ferrooxidans

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
46000	-	SDS-PAGE	Acidithiobacillus ferrooxidans

Organism

Organism	UniProt	Comment	Textmining
Acidithiobacillus ferrooxidans	-	strain ATCC 23270	-

Purification (Commentary)

Purification (Comment)	Organism
Hi-Trap column chromatography	Acidithiobacillus ferrooxidans

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
0.0224	-	crude extract, at 25°C	Acidithiobacillus ferrooxidans
0.148	-	enzyme after purification, at 25°C	Acidithiobacillus ferrooxidans

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
L-cysteine + [enzyme]-cysteine	IscS has a high affinity for L-cysteine	Acidithiobacillus ferrooxidans	L-alanine + [enzyme]-S-sulfanylcysteine	-	?

Synonyms

Synonyms	Comment	Organism
IscS	-	Acidithiobacillus ferrooxidans

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
30	-	-	Acidithiobacillus ferrooxidans

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
40	55	the enzyme is relatively stable at 40°C with 67% loss of activity, and unstable at temperatures above 55°C	Acidithiobacillus ferrooxidans

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
8	8.5	-	Acidithiobacillus ferrooxidans

pH Range

pH Minimum	pH Maximum	Comment	Organism
7	9	-	Acidithiobacillus ferrooxidans

pH Stability

pH Stability	pH Stability Maximum	Comment	Organism
5	10	about 45% residual activity at pH 7.5 and 9.5, almost no activity at pH 5.0 and 10.0	Acidithiobacillus ferrooxidans

Cofactor

Cofactor	Comment	Organism	Structure
pyridoxal 5'-phosphate	-	Acidithiobacillus ferrooxidans

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Release 2023.1 (January 2023)