Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli C41 cells | Archaeoglobus fulgidus |
Crystallization (Comment) | Organism |
---|---|
hanging drop vapor diffusion method, using 0.2 M calcium acetate, 0.1 sodium cacodylate pH 6.5, 40% (v/v) PEG 300 | Archaeoglobus fulgidus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Archaeoglobus fulgidus | O29689 | - |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation, column chromatography, and Superose 12 gel filtration | Archaeoglobus fulgidus |
Synonyms | Comment | Organism |
---|---|---|
IscS | - |
Archaeoglobus fulgidus |
L-cysteine desulfurase | - |
Archaeoglobus fulgidus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | adding pyridoxal 5'-phosphate to IscS produces an enzyme that displays in vitro L-cysteine desulfurase activity | Archaeoglobus fulgidus | |
pyridoxamine 5'-phosphate | the enzyme contains pyridoxamine 5'-phosphate which is catalytically inactive in the cysteine desulfurase reaction, leading to a lack of cysteine desulfurase activity | Archaeoglobus fulgidus |