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Literature summary for 2.8.1.7 extracted from

  • Blauenburg, B.; Mielcarek, A.; Altegoer, F.; Fage, C.D.; Linne, U.; Bange, G.; Marahiel, M.A.
    Crystal structure of Bacillus subtilis cysteine desulfurase SufS and its dynamic interaction with frataxin and scaffold protein SufU (2016), PLoS ONE, 11, e0158749.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.8.1.7

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Bacillus subtilis

Crystallization (Commentary)

Crystallization (Comment) Organism
homodimer in complex with pyrodoxal 5'-phosphate, alanine, and Cys361-persulfide, sitting drop vapor diffusion method, using 0.1 M HEPES, pH 7.5, 50% (w/v) PEG 400 Bacillus subtilis

Localization

Localization Comment Organism GeneOntology No. Textmining

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-cysteine + acceptor Bacillus subtilis
-
 L-alanine + S-sulfanyl-acceptor
-
 ?

Organism

Organism UniProt Comment Textmining
Bacillus subtilis O32164
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni affinity column chromatography and Superdex 200 gel filtration Bacillus subtilis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-cysteine + acceptor
-
 Bacillus subtilis L-alanine + S-sulfanyl-acceptor
-
 ?

Subunits

Subunits Comment Organism
homodimer x-ray crystallography Bacillus subtilis

Synonyms

Synonyms Comment Organism
SufS
-
 Bacillus subtilis

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate
-
 Bacillus subtilis