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Literature summary for 2.8.2.1 extracted from

  • Yasuda, S.; Kumar, A.P.; Liu, M.Y.; Sakakibara, Y.; Suiko, M.; Chen, L.; Liu, M.C.
    Identification of a novel thyroid hormone-sulfating cytosolic sulfotransferase, SULT1 ST5, from zebrafish (2005), FEBS J., 272, 3828-3837.
    View publication on PubMed
Search for more literature for 2.8.2.1

Cloned(Commentary)

Cloned (Comment) Organism
recombinant enzyme form SULT1 ST5 is expressed using the pGEX-2TK bacterial expression system, expression in BL21 (DE3) cells Danio rerio

Inhibitors

Inhibitors Comment Organism Structure
Cd2+ recombinant enzyme form SULT1 ST5 Danio rerio
Co2+ recombinant enzyme form SULT1 ST5 Danio rerio
Cu2+ recombinant enzyme form SULT1 ST5 Danio rerio
Fe2+ recombinant enzyme form SULT1 ST5 Danio rerio
Hg2+ recombinant enzyme form SULT1 ST5 Danio rerio
Pb2+ recombinant enzyme form SULT1 ST5 Danio rerio
Zn2+ recombinant enzyme form SULT1 ST5 Danio rerio

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0277
-
 3,3',5-triiodo-D-thyronine pH 8.0, 28°C, enzyme form SULT1 ST5 Danio rerio
0.0387
-
 3,3',5-triiodo-L-thyronine pH 8.0, 28°C, enzyme form SULT1 ST5 Danio rerio
0.0445
-
 L-thyroxine pH 8.0, 28°C, enzyme form SULT1 ST5 Danio rerio
0.0977
-
 beta-naphthol pH 8.0, 28°C, enzyme form SULT1 ST5 Danio rerio

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
 Danio rerio 5829
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+ significant stimulation of enzyme form SULT1 ST5 Danio rerio

Organism

Organism UniProt Comment Textmining
Danio rerio
-
 recombinant enzyme form SULT1 ST5
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme form SULT1 ST5 expressed in BL21 (DE3) cells Danio rerio

Source Tissue

Source Tissue Comment Organism Textmining
embryo significant level of expression at the beginning of the hatching period during embryogenesis, enzyme form SULT1 ST5 Danio rerio
-
larva gradual increase in expression to a high level of expression throughout the larval stage of maturity, enzyme form SULT1 ST5 Danio rerio
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3'-phosphoadenylylsulfate + 2-naphthol recombinant enzyme form SULT1 ST5 Danio rerio adenosine 3',5'-bisphosphate + 2-naphthylsulfate
-
 ?
3'-phosphoadenylylsulfate + 3,3',5-triiodo-D-thyronine recombinant enzyme form SULT1 ST5 Danio rerio adenosine 3',5'-bisphosphate + 3,3',5-triiodo-D-thyronine 4'-sulfate
-
 ?
3'-phosphoadenylylsulfate + 3,3',5-triiodo-L-thyronine recombinant enzyme form SULT1 ST5 Danio rerio adenosine 3',5'-bisphosphate + 3,3',5-triiodo-L-thyronine 4'-sulfate
-
 ?
3'-phosphoadenylylsulfate + 4-nitrophenol recombinant enzyme form SULT1 ST5 Danio rerio adenosine 3',5'-bisphosphate + 4-nitrophenyl sulfate
-
 ?
3'-phosphoadenylylsulfate + butylated hydroxyanisole recombinant enzyme form SULT1 ST5 Danio rerio adenosine 3',5'-bisphosphate + ?
-
 ?
3'-phosphoadenylylsulfate + caffeic acid recombinant enzyme form SULT1 ST5 Danio rerio adenosine 3',5'-bisphosphate + ?
-
 ?
3'-phosphoadenylylsulfate + catechin recombinant enzyme form SULT1 ST5 Danio rerio adenosine 3',5'-bisphosphate + ?
-
 ?
3'-phosphoadenylylsulfate + chlorogenic acid recombinant enzyme form SULT1 ST5 Danio rerio adenosine 3',5'-bisphosphate + ?
-
 ?
3'-phosphoadenylylsulfate + daidzein recombinant enzyme form SULT1 ST5 Danio rerio adenosine 3',5'-bisphosphate + ?
-
 ?
3'-phosphoadenylylsulfate + gallic acid recombinant enzyme form SULT1 ST5 Danio rerio adenosine 3',5'-bisphosphate + ?
-
 ?
3'-phosphoadenylylsulfate + genistein recombinant enzyme form SULT1 ST5 Danio rerio adenosine 3',5'-bisphosphate + ?
-
 ?
3'-phosphoadenylylsulfate + kaempferol recombinant enzyme form SULT1 ST5 Danio rerio adenosine 3',5'-bisphosphate + ?
-
 ?
3'-phosphoadenylylsulfate + L-thyroxine recombinant enzyme form SULT1 ST5 Danio rerio adenosine 3',5'-bisphosphate + L-thyroxine 4'-sulfate
-
 ?
3'-phosphoadenylylsulfate + myricetin recombinant enzyme form SULT1 ST5 Danio rerio adenosine 3',5'-bisphosphate + ?
-
 ?
3'-phosphoadenylylsulfate + n-propyl gallate recombinant enzyme form SULT1 ST5 Danio rerio adenosine 3',5'-bisphosphate + ?
-
 ?
3'-phosphoadenylylsulfate + quercetin recombinant enzyme form SULT1 ST5 Danio rerio adenosine 3',5'-bisphosphate + ?
-
 ?

Synonyms

Synonyms Comment Organism
SULT1 ST5
-
 Danio rerio

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
 with beta-naphthol as substrate, two optima at pH 6.0 and at pH 9.0 with 3,3',5-triiodo-L-thyronine as substrate, enzyme form SULT1 ST5 Danio rerio
9
-
 two optima at pH 6.0 and at pH 9.0 with 3,3',5-triiodo-L-thyronine as substrate, enzyme form SULT1 ST5 Danio rerio