Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cd2+ | strongly inhibited by 5 mM | Rattus norvegicus | |
corticosterone | competitive inhibitor, inhibits cortisol sulfotransferase activity | Rattus norvegicus | |
cortisol | - |
Rattus norvegicus | |
cortisone | - |
Rattus norvegicus | |
dehydroepiandrosterone | competitive inhibitor, inhibits cortisol sulfotransferase activity | Rattus norvegicus | |
deoxycorticosterone | competitive inhibitor, inhibits cortisol sulfotransferase activity | Rattus norvegicus | |
estradiol-17beta | competitive inhibitor, inhibits cortisol sulfotransferase activity | Rattus norvegicus | |
additional information | unaffected by 8 mM ADP, ATP, and AMP, diethylstilbestrol, cortisone and cholesterol are no inhibitors for the purified enzyme | Rattus norvegicus | |
p-hydroxymercuribenzoate | - |
Rattus norvegicus | |
progesterone | competitive inhibitor, inhibits cortisol sulfotransferase activity | Rattus norvegicus | |
testosterone | competitive inhibitor, inhibits cortisol sulfotransferase activity | Rattus norvegicus | |
Zn2+ | strongly inhibited by 5 mM | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00628 | - |
3'-phosphoadenylylsulfate | pH 6.8, 37.5°C | Rattus norvegicus | |
0.00682 | - |
cortisol | pH 6.8, 37.5°C | Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | - |
Rattus norvegicus | 5737 | - |
cytosol | - |
Rattus norvegicus | 5829 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ba2+ | markedly activated by 5 mM divalent cations | Rattus norvegicus | |
Ca2+ | markedly activated by 5 mM divalent cations | Rattus norvegicus | |
Co2+ | markedly activated by 5 mM divalent cations | Rattus norvegicus | |
Cr2+ | markedly activated by 5 mM divalent cations | Rattus norvegicus | |
Mg2+ | markedly activated by 5 mM divalent cations | Rattus norvegicus | |
Mn2+ | most effective, markedly activated by 5 mM divalent cations | Rattus norvegicus | |
Ni2+ | markedly activated by 5 mM divalent cations | Rattus norvegicus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
61500 | - |
gel filtration | Rattus norvegicus |
65000 | - |
sedimentation velocity experiment in preparative ultracentrifuge | Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
3'-phosphoadenylylsulfate + dehydroepiandrosterone | Rattus norvegicus | enzymatic sulfation of steroids | adenosine 3',5'-bisphosphate + dehydroepiandrosterone 3-sulfate | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
rat | - |
Rattus norvegicus | - |
CDR Fisher, male | - |
Purification (Comment) | Organism |
---|---|
partial | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.000978 | - |
- |
Rattus norvegicus |
Storage Stability | Organism |
---|---|
-20°C, partially purified enzyme, stable for at least 3 months | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3'-phosphoadenylylsulfate + 17beta-estradiol | - |
Rattus norvegicus | adenosine 3',5'-bisphosphate + ? | - |
r | |
3'-phosphoadenylylsulfate + cortisol | - |
Rattus norvegicus | adenosine 3',5'-bisphosphate + cortisol sulfate | - |
r | |
3'-phosphoadenylylsulfate + dehydroepiandrosterone | - |
Rattus norvegicus | adenosine 3',5'-bisphosphate + dehydroepiandrosterone 3-sulfate | - |
r | |
3'-phosphoadenylylsulfate + dehydroepiandrosterone | enzymatic sulfation of steroids | Rattus norvegicus | adenosine 3',5'-bisphosphate + dehydroepiandrosterone 3-sulfate | - |
r | |
3'-phosphoadenylylsulfate + testosterone | - |
Rattus norvegicus | adenosine 3',5'-bisphosphate + testosterone sulfate | - |
r |
Synonyms | Comment | Organism |
---|---|---|
STIII | - |
Rattus norvegicus |
sulfotransferase III | - |
Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
- |
Rattus norvegicus |