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Literature summary for 2.8.2.24 extracted from
- Partial purification and characterization of a 3'-phosphoadenosine 5'-phosphosulfate: desulfoglucosinolate sulfotransferase from cress (Lepidium sativum) (1990), Plant Physiol., 94, 811-816.
General Stability
| General Stability | Organism |
|---|---|
| bovine serum albumin, required for stabilizing | Lepidium sativum |
| dilution inactivates | Lepidium sativum |
| MgCl2, 2-mercaptoethanol, DTT and GSH do not stabilize | Lepidium sativum |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2,2'-dipyridyl | 26% inhibition at 10 mM | Lepidium sativum |  |
| adenosine-3',5'-bisphosphate | product inhibition | Lepidium sativum | |
| Ag+ | 13% inhibition at 1 mM | Lepidium sativum | |
| Cu2+ | 14% inhibition at 1 mM | Lepidium sativum | |
| DTNB | 2-mercaptoethanol protects | Lepidium sativum | |
| EDTA | 15% inhibition at 10 mM | Lepidium sativum | |
| iodoacetamide | weak inhibition | Lepidium sativum | |
| iodoacetic acid | weak inhibition | Lepidium sativum | |
| additional information | no inhibition by FeCl3, FeCl2, CaCl2, CoCl2 | Lepidium sativum | |
| N-ethylmaleimide | 2-mercaptoethanol protects | Lepidium sativum | |
| Ni2+ | 30% inhibition at 1 mM | Lepidium sativum | |
| o-phenanthroline | 34% inhibition at 10 mM | Lepidium sativum | |
| p-chloromercuriphenylsulfonic acid | 2-mercaptoethanol protects | Lepidium sativum | |
| Pb(NO3)2 | 25% inhibition at 1 mM | Lepidium sativum | |
| Zn2+ | 46% inhibition at 1 mM | Lepidium sativum | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | kinetic studies | Lepidium sativum | |
| 0.0065 | - |
desulfoallylglucosinolate | pH 9.0, 30°C | Lepidium sativum | |
| 0.06 | - |
3'-phosphoadenylylsulfate | pH 9.0, 30°C | Lepidium sativum | |
| 0.082 | - |
desulfobenzylglucosinolate | pH 9.0, 30°C | Lepidium sativum | |
| 0.67 | - |
desulfo-p-hydroxy-benzylglucosinolate | pH 9.0, 30°C | Lepidium sativum | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | 51% activation at 10 mM | Lepidium sativum | |
| Mn2+ | 23% activation at 1 mM, 10 mM inhibits by 53% | Lepidium sativum | |
| additional information | not affected by FeCl3, FeCl2, CaCl2, CoCl2 | Lepidium sativum |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 31000 | - |
gel filtration | Lepidium sativum |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3'-phosphoadenylylsulfate + desulfobenzylglucosinolate | Lepidium sativum | - |
adenosine 3',5'-bisphosphate + benzylglucosinolate | - |
? | |
| additional information | Lepidium sativum | involved in the biosynthesis of glucosinolates | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Arabidopsis thaliana | - |
- |
- |
| Brassica rapa subsp. oleifera | - |
- |
- |
| Lepidium sativum | - |
no.5089, curled cress | - |
| Sinapis alba | - |
- |
- |
| Tropaeolum majus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| 40.1fold | Lepidium sativum |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| 3'-phosphoadenylyl sulfate + desulfoglucotropeolin = adenosine 3',5'-bisphosphate + glucotropeolin | mechanism | Lepidium sativum |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| seedling | etiolated | Arabidopsis thaliana | - |
| seedling | etiolated | Lepidium sativum | - |
| seedling | etiolated | Brassica rapa subsp. oleifera | - |
| seedling | etiolated | Sinapis alba | - |
| seedling | etiolated | Tropaeolum majus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.935 | - |
purified enzyme | Lepidium sativum |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, 0.25 M sucrose and bovine serum albumin, 6 months | Lepidium sativum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3'-phosphoadenylyl sulfate + desulfo-p-hydroxy-benzylglucosinolate | - |
Lepidium sativum | adenosine 3',5'-bisphosphate + p-hydroxy-benzylglucosinolate | - |
? | |
| 3'-phosphoadenylyl sulfate + desulfoallylglucosinolate | - |
Lepidium sativum | adenosine 3',5'-bisphosphate + allylglucosinolate | - |
? | |
| 3'-phosphoadenylyl sulfate + desulfobenzylglucosinolate | i.e. 3'-phosphoadenosine 5'phosphosulfate, i.e. PAPS | Lepidium sativum | adenosine 3',5'-bisphosphate + benzylglucosinolate | - |
? | |
| 3'-phosphoadenylyl sulfate + desulfobenzylglucosinolate | absolute specificity for desulfoglucosinolate structure | Arabidopsis thaliana | adenosine 3',5'-bisphosphate + benzylglucosinolate | - |
? | |
| 3'-phosphoadenylyl sulfate + desulfobenzylglucosinolate | absolute specificity for desulfoglucosinolate structure | Lepidium sativum | adenosine 3',5'-bisphosphate + benzylglucosinolate | - |
? | |
| 3'-phosphoadenylyl sulfate + desulfobenzylglucosinolate | absolute specificity for desulfoglucosinolate structure | Brassica rapa subsp. oleifera | adenosine 3',5'-bisphosphate + benzylglucosinolate | - |
? | |
| 3'-phosphoadenylyl sulfate + desulfobenzylglucosinolate | absolute specificity for desulfoglucosinolate structure | Sinapis alba | adenosine 3',5'-bisphosphate + benzylglucosinolate | - |
? | |
| 3'-phosphoadenylyl sulfate + desulfobenzylglucosinolate | absolute specificity for desulfoglucosinolate structure | Tropaeolum majus | adenosine 3',5'-bisphosphate + benzylglucosinolate | - |
? | |
| 3'-phosphoadenylylsulfate + desulfobenzylglucosinolate | - |
Lepidium sativum | adenosine 3',5'-bisphosphate + benzylglucosinolate | - |
? | |
| additional information | no acceptors are quercetin, rutin, kaempferol, p-coumaric acid, ferulic acid, caffeic acid, phenylacetaldoxime | Lepidium sativum | ? | - |
? | |
| additional information | involved in the biosynthesis of glucosinolates | Lepidium sativum | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 31000-55000, SDS-PAGE | Lepidium sativum |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| ST | - |
Lepidium sativum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 9 | - |
maximal activity in Tris-HCl buffer | Lepidium sativum |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 9.5 | about 65% of maximal activity at pH 7.0 and pH 9.5 | Lepidium sativum |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| additional information | - |
activity loss due to instability at pH values near the isoelectric point 5.2 | Lepidium sativum |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Lepidium sativum | - |
- |
5.2 |
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