Inhibitors | Comment | Organism | Structure |
---|---|---|---|
adenosine-3',5'-bisphosphate | competitive to 3'-phosphoadenylylsulfate, kinetics | Brassica juncea | |
Co2+ | at low concentration | Brassica juncea | |
Cu2+ | at low concentration | Brassica juncea | |
iodoacetamide | - |
Brassica juncea | |
iodoacetic acid | weak inhibition | Brassica juncea | |
Iodosobenzoic acid | - |
Brassica juncea | |
Mn2+ | inhibitory at 10 mM, stimulating at 1 mM | Brassica juncea | |
additional information | no inhibition by 3'-AMP, 5'-AMP, 2',5'-AMP, 3',5'-cAMP, 5'-ADP, 5'-ATP, adenosine-5'-phosphosulfate, benzaldoxime, phenylacetohydroximate, phenylacetothiohydroximate, S-methylphenylacetothiohydroximate, benzylglucosinolate | Brassica juncea | |
N-Methylmaleimide | - |
Brassica juncea | |
N-Pyrenylmaleimide | - |
Brassica juncea | |
PCMB | - |
Brassica juncea | |
Phenylmercuriacetate | - |
Brassica juncea | |
Zn2+ | at low concentration | Brassica juncea |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00078 | - |
3'-phosphoadenylylsulfate | pH 8.5-9.0, 30°C | Brassica juncea | |
0.0023 | - |
desulfobenzylglucosinolate | pH 8.5-9.0, 30°C | Brassica juncea |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | slight activation | Brassica juncea | |
Mn2+ | 23% activation at 1 mM, 10 mM inhibits by 53% | Brassica juncea | |
additional information | no absolute requirement for cations | Brassica juncea |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
44000 | - |
gel filtration | Brassica juncea |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
3'-phosphoadenylylsulfate + desulfobenzylglucosinolate | Brassica juncea | - |
adenosine 3',5'-bisphosphate + benzylglucosinolate | - |
? | |
additional information | Brassica juncea | involved in the biosynthesis of glucosinolates | ? | - |
? | |
additional information | Brassica juncea | involved with EC 2.4.1.195 in final steps of thioglycoside biosynthesis in cruciferous plants | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Brassica juncea | - |
cv. Cutlass | - |
Purification (Comment) | Organism |
---|---|
partial, 230fold | Brassica juncea |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cell culture | derived from hypocotyls | Brassica juncea | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.0000329 | - |
partially purified enzyme | Brassica juncea |
Storage Stability | Organism |
---|---|
-20°C, 20 mM Tris buffer, pH 7.5, 14 mM 2-mercaptoethanol, 10% glycerol, at least 2 months | Brassica juncea |
4°C, 14 mM 2-mercaptoethanol, t1/2: about 2 weeks | Brassica juncea |
4°C, more than 50% loss of activity within 48 h | Brassica juncea |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3'-phosphoadenylyl sulfate + desulfobenzylglucosinolate | absolute specificity for desulfoglucosinolate structure | Brassica juncea | adenosine 3',5'-bisphosphate + benzylglucosinolate | - |
? | |
3'-phosphoadenylylsulfate + desulfobenzylglucosinolate | - |
Brassica juncea | adenosine 3',5'-bisphosphate + benzylglucosinolate | - |
? | |
additional information | no activity with adenosine-5'-phosphosulfate as donor | Brassica juncea | ? | - |
? | |
additional information | involved in the biosynthesis of glucosinolates | Brassica juncea | ? | - |
? | |
additional information | involved with EC 2.4.1.195 in final steps of thioglycoside biosynthesis in cruciferous plants | Brassica juncea | ? | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
- |
Brassica juncea |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | 40 | about 85% of maximal activity at 25°C and about half-maximal activity at 40°C | Brassica juncea |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
at least 1 h, inactivation above | Brassica juncea |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | 9 | maximal activity in Tris-HCl buffer | Brassica juncea |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0004 | - |
adenosine-3',5'-bisphosphate | pH 8.5-9.0, 30°C | Brassica juncea |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Brassica juncea | - |
- |
4.84 |