Literature summary for 2.8.2.34 extracted from

Barnes, S.; Buchina, E.S.; King, R.J.; McBurnett, T.; Taylor, K.B.
Bile acid sulfotransferase I from rat liver sulfates bile acids and 3-hydroxy steroids: purification, N-terminal amino acid sequence, and kinetic properties (1989), J. Lipid Res., 30, 529-540.

Search for more literature for 2.8.2.34

Inhibitors

Inhibitors	Comment	Organism	Structure
3,6-dioxo-5alpha-cholanoate	-	Rattus norvegicus
3,6-dioxo-5beta-cholanoate	-	Rattus norvegicus
3,7-dioxo-5beta-cholanoate	-	Rattus norvegicus
3-oxo-5beta-cholanic acid	80-90% inhibition at equimolar amounts	Rattus norvegicus
3alpha,6beta-dihydroxy-5beta-cholanoate	-	Rattus norvegicus
6-oxolithocholate	-	Rattus norvegicus
7-oxolithocholate	-	Rattus norvegicus
chenodeoxycholate	-	Rattus norvegicus
hyodeoxycholate	-	Rattus norvegicus
additional information	no inhibition by 3,12-dioxo-5beta-cholanoate	Rattus norvegicus
ursodeoxycholate	-	Rattus norvegicus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
additional information	-	additional information	hyperbolic kinetics, allosteric effect	Rattus norvegicus
0.00024	-	3beta-hydroxy-5beta-cholanoic acid	pH 6.5, 37°C, 5 mM MgCl2	Rattus norvegicus
0.00072	-	dehydroepiandrosterone	pH 6.5, 37°C, 5 mM MgCl2	Rattus norvegicus

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
cytosol	-	Rattus norvegicus	5829	-

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	maximal activity at 5 mM MgCl2, Mg2+ is not essential for activity	Rattus norvegicus
NaCl	induces aggregation of the purified enzyme at 0.5 M	Rattus norvegicus

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
additional information	-	the enzyme shows dfferent higher aggregation forms	Rattus norvegicus
30000	-	x * 30000, SDS-PAGE	Rattus norvegicus

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
additional information	Rattus norvegicus	the enzyme is involved in prevention of toxicity of monohydroxy bile acids	?	-	?

Organism

Organism	UniProt	Comment	Textmining
Rattus norvegicus	-	female Sprague-Dawley rats	-

Purification (Commentary)

Purification (Comment)	Organism
157fold by ultracentrifugation, ion exchange chromatography, and adenosine 3',5'-bisphosphate affinity chromatpgraphy	Rattus norvegicus

Reaction

Reaction	Comment	Organism	Reaction ID
3'-phosphoadenylyl sulfate + glycochenodeoxycholate = adenosine 3',5'-bisphosphate + glycochenodeoxycholate 7-sulfate	the enzyme is identical with the hydrosteroid sulfotransferase 2	Rattus norvegicus	a

Source Tissue

Source Tissue	Comment	Organism	Textmining
liver	-	Rattus norvegicus	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
0.0187	-	purified enzyme	Rattus norvegicus

Storage Stability

Storage Stability	Organism
4°C, 0.1 mg/ml purified enzyme, 3 weeks, no loss of activity	Rattus norvegicus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
3'-phosphoadenylyl 5'-phosphosulfate + 3beta-hydroxy-5beta-cholanoic acid	-	Rattus norvegicus	adenosine 3',5'-bisphosphate + 3beta-hydroxy-5beta-cholanoic acid 3-sulfate	-	?
3'-phosphoadenylyl 5'-phosphosulfate + chenodeoxycholate	low activity	Rattus norvegicus	adenosine 3',5'-bisphosphate + chenodeoxycholate 3-sulfate	-	?
3'-phosphoadenylyl 5'-phosphosulfate + cortisol	low activity	Rattus norvegicus	adenosine 3',5'-bisphosphate + ?	-	?
3'-phosphoadenylyl 5'-phosphosulfate + dehydroepiandrosterone	i.e. androst-5-ene-3beta-ol-17-one, best substrate	Rattus norvegicus	adenosine 3',5'-bisphosphate + dehydroepiandrosterone 3-sulfate	-	?
3'-phosphoadenylyl 5'-phosphosulfate + deoxycholate	i.e. 3alpha,12alpha-dihydroxy-5beta-cholan-24-oic acid, low activity	Rattus norvegicus	adenosine 3',5'-bisphosphate + deoxycholate 3-sulfate	-	?
3'-phosphoadenylyl 5'-phosphosulfate + estradiol	low activity	Rattus norvegicus	adenosine 3',5'-bisphosphate + ?	-	?
3'-phosphoadenylyl 5'-phosphosulfate + glycochenodeoxycholate	low activity	Rattus norvegicus	adenosine 3',5'-bisphosphate + ?	-	?
3'-phosphoadenylyl 5'-phosphosulfate + glycodeoxycholate	-	Rattus norvegicus	adenosine 3',5'-bisphosphate + ?	-	?
3'-phosphoadenylyl 5'-phosphosulfate + glycolithocholate	low activity	Rattus norvegicus	adenosine 3',5'-bisphosphate + glycolithocholate 3-sulfate	-	?
3'-phosphoadenylyl 5'-phosphosulfate + glycolithocholate	i.e. N-(3-hydroxy-5beta-cholanoyl)glycine	Rattus norvegicus	adenosine 3',5'-bisphosphate + glycolithocholate 3-sulfate	-	?
3'-phosphoadenylyl 5'-phosphosulfate + lithocholate	low activity	Rattus norvegicus	adenosine 3',5'-bisphosphate + lithocholate 3-sulfate	-	?
3'-phosphoadenylyl 5'-phosphosulfate + taurochenodeoxycholate	low activity	Rattus norvegicus	adenosine 3',5'-bisphosphate + ?	-	?
3'-phosphoadenylyl 5'-phosphosulfate + taurodeoxycholate	-	Rattus norvegicus	adenosine 3',5'-bisphosphate + ?	-	?
3'-phosphoadenylyl 5'-phosphosulfate + taurolithocholate	low activity	Rattus norvegicus	adenosine 3',5'-bisphosphate + taurolithocholate 3-sulfate	-	?
3'-phosphoadenylyl 5'-phosphosulfate + testosterone	low activity	Rattus norvegicus	adenosine 3',5'-bisphosphate + ?	-	?
additional information	the enzyme is involved in prevention of toxicity of monohydroxy bile acids	Rattus norvegicus	?	-	?
additional information	substrate specificity, enzyme is most active with bile acids or steroids possessing a 3beta-hydroxy group and a 5-6 double bond or a trans A-B ring junction at the steroid nucleus, poor or no activity with cholate and conjugates, ursodeoxycholate, hyodeoxycholate, 3alpha,6alpha-dihydroxy-5beta-cholanoate, overview	Rattus norvegicus	?	-	?

Subunits

Subunits	Comment	Organism
?	x * 30000, SDS-PAGE	Rattus norvegicus

Synonyms

Synonyms	Comment	Organism
BAST I	-	Rattus norvegicus
bile acid sulfotransferase I	-	Rattus norvegicus
bile acid:3'phosphoadenosine-5'phosphosulfate:sulfotransferase	-	Rattus norvegicus
More	the enzyme is identical with the hydrosteroid sulfotransferase 2	Rattus norvegicus

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
37	-	assay at	Rattus norvegicus

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
6.5	-	at 5 mM Mg2+, broad optimum at pH 5.5-7.5	Rattus norvegicus

pH Range

pH Minimum	pH Maximum	Comment	Organism
5	8	-	Rattus norvegicus

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
0.26	-	3-oxo-5beta-cholanic acid	pH 6.5, 37°C, 5 mM MgCl2	Rattus norvegicus

pI Value

Organism	Comment	pI Value Maximum	pI Value
Rattus norvegicus	isoelectric focusing	-	5.3

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Release 2023.1 (January 2023)