Crystallization (Comment) | Organism |
---|---|
- |
Acidaminococcus fermentans |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
4-hydroxymercuribenzoate | 0.2 mM, at 37°C, 2 min, 80% loss of activity, 2-mercaptoethanol partially restores activity | Acidaminococcus fermentans | |
additional information | not inhibited by 2 mM iodoacetate, 2 mM iodoacetamide or 1 mM 5,5-dithiobis(2-nitrobenzoate) | Acidaminococcus fermentans | |
NaBH4 | 10 mM, in presence of 0.4 mM glutyral-CoA, at 25°C, 30 min, almost complete inhibition | Acidaminococcus fermentans |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.015 | - |
glutaryl-CoA | pH 7, 25°C, cosubstrate acetate | Acidaminococcus fermentans | |
0.017 | - |
glutaconyl-CoA | cosubstrate glutarate | Acidaminococcus fermentans | |
0.17 | - |
acetyl-CoA | cosubstrate glutaconate | Acidaminococcus fermentans | |
0.2 | - |
glutaconate | pH 7, 25°C, cosubstrate acetyl-CoA | Acidaminococcus fermentans | |
0.7 | - |
Glutarate | cosubstrate glutaconyl-CoA | Acidaminococcus fermentans | |
1.5 | - |
(R)-2-hydroxyglutarate | cosubstrate glutaconyl-CoA | Acidaminococcus fermentans | |
8 | - |
adipate | cosubstrate glutaconyl-CoA | Acidaminococcus fermentans | |
10 | - |
Acrylate | cosubstrate acetyl-CoA | Acidaminococcus fermentans | |
13 | - |
3-hydroxyglutarate | cosubstrate glutaconyl-CoA | Acidaminococcus fermentans | |
14 | - |
(S)-2-hydroxyglutarate | cosubstrate glutaconyl-CoA | Acidaminococcus fermentans | |
16 | - |
propionate | cosubstrate glutaconyl-CoA | Acidaminococcus fermentans | |
26 | - |
acetate | cosubstrate glutaconyl-CoA | Acidaminococcus fermentans | |
100 | - |
isocrotonate | cosubstrate acetyl-CoA | Acidaminococcus fermentans | |
150 | - |
Butyrate | cosubstrate glutaconyl-CoA | Acidaminococcus fermentans | |
500 | - |
crotonate | cosubstrate acetyl-CoA | Acidaminococcus fermentans |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
32000 | - |
alpha4beta4, 4 * 32000 + 4 * 34000, SDS-PAGE | Acidaminococcus fermentans |
34000 | - |
alpha4beta4, 4 * 32000 + 4 * 34000, SDS-PAGE | Acidaminococcus fermentans |
275000 | - |
gel filtration | Acidaminococcus fermentans |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + (E)-glutaconate | Acidaminococcus fermentans | involved in glutamate fermentation via hydroxyglutarate pathway, activates glutaconate to glutaconyl-CoA prior to glutaconate decarboxylation | acetate + glutaconyl-1-CoA | - |
? | |
additional information | Acidaminococcus fermentans | not involved in the dehydration of (R)-2-hydroxyglutarate to glutaconate | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Acidaminococcus fermentans | - |
ATCC 25085 | - |
no activity in Clostridium tetanomorphum | - |
- |
- |
no activity in Clostridium tetanomorphum H1 | - |
- |
- |
[Clostridium] sporosphaeroides | - |
NCIB 10672 | - |
[Clostridium] symbiosum | - |
HB 25 | - |
[Clostridium] symbiosum HB 25 | - |
HB 25 | - |
Purification (Comment) | Organism |
---|---|
- |
Acidaminococcus fermentans |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
acetyl-CoA + (E)-glutaconate = acetate + glutaconyl-1-CoA | ping-pong mechanism | Acidaminococcus fermentans |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
[Clostridium] sporosphaeroides |
additional information | - |
- |
[Clostridium] symbiosum |
additional information | - |
- |
Acidaminococcus fermentans |
Storage Stability | Organism |
---|---|
4°C, crystalline suspension in ammonium sulfate, 2 years, stable | Acidaminococcus fermentans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + (E)-glutaconate | - |
[Clostridium] sporosphaeroides | acetate + glutaconyl-1-CoA | - |
? | |
acetyl-CoA + (E)-glutaconate | - |
[Clostridium] symbiosum | acetate + glutaconyl-1-CoA | - |
? | |
acetyl-CoA + (E)-glutaconate | very good substrate, broad substrate specificity | Acidaminococcus fermentans | acetate + glutaconyl-1-CoA | - |
? | |
acetyl-CoA + (E)-glutaconate | very good substrate, broad substrate specificity | Acidaminococcus fermentans | acetate + glutaconyl-1-CoA | - |
r | |
acetyl-CoA + (E)-glutaconate | removal of 3'-phospho group from acetyl-CoA leads to reduced activity | Acidaminococcus fermentans | acetate + glutaconyl-1-CoA | - |
? | |
acetyl-CoA + (E)-glutaconate | removal of 3'-phospho group from acetyl-CoA leads to reduced activity | Acidaminococcus fermentans | acetate + glutaconyl-1-CoA | - |
r | |
acetyl-CoA + (E)-glutaconate | involved in glutamate fermentation via hydroxyglutarate pathway, activates glutaconate to glutaconyl-CoA prior to glutaconate decarboxylation | Acidaminococcus fermentans | acetate + glutaconyl-1-CoA | - |
? | |
acetyl-CoA + (E)-glutaconate | - |
[Clostridium] symbiosum HB 25 | acetate + glutaconyl-1-CoA | - |
? | |
acetyl-CoA + (R)-2-hydroxyglutarate | - |
Acidaminococcus fermentans | acetate + (R)-2-hydroxyglutaryl-1-CoA | in vitro formation of both isomers: (R)-2-hydroxyglutaryl-1-CoA and (R)-2-hydroxyglutaryl-5-CoA, the 1-isomer is kinetically favoured, the 5-isomer is thermodynamically favoured | ? | |
acetyl-CoA + acrylate | poor substrate | Acidaminococcus fermentans | acrylyl-CoA + acetate | - |
? | |
acetyl-CoA + crotonate | poor substrate | Acidaminococcus fermentans | crotonyl-CoA + acetate | - |
? | |
acetyl-CoA + isocrotonate | poor substrate | Acidaminococcus fermentans | isocrotonyl-CoA + acetate | - |
? | |
glutaconyl-CoA + (R)-2-hydroxyglutarate | very good substrate | Acidaminococcus fermentans | glutaconate + (R)-2-hydroxyglutaryl-CoA | generation of both isomers: (R)-2-hydroxyglutaryl-1-CoA and (R)-2-hydroxyglutaryl-5-CoA | ? | |
glutaconyl-CoA + (S)-2-hydroxyglutarate | at 60% of the rate with (R)-isomer or glutarate | Acidaminococcus fermentans | glutaconate + (S)-2-hydroxyglutaryl-CoA | - |
? | |
glutaconyl-CoA + 3-hydroxyglutarate | at 20% of the rate with glutarate | Acidaminococcus fermentans | glutaconate + 3-hydroxyglutaryl-CoA | - |
? | |
glutaconyl-CoA + adipate | at 60% of the rate with glutarate | Acidaminococcus fermentans | glutaconate + adipyl-CoA | - |
? | |
glutaconyl-CoA + butyrate | - |
Acidaminococcus fermentans | butyryl-CoA + glutaconate | - |
? | |
glutaconyl-CoA + glutarate | very good substrate | Acidaminococcus fermentans | glutaconate + glutaryl-CoA | - |
? | |
glutaconyl-CoA + propionate | moderate substrate | Acidaminococcus fermentans | propanoyl-CoA + glutaconate | - |
? | |
glutaryl-CoA + acetate | - |
Acidaminococcus fermentans | glutarate + acetyl-CoA | - |
r | |
additional information | no substrates: (Z)-glutaconate, acetyl-4'-phosphopantetheine, C4-dicarboxylic acids, reverse reaction not with glutamate, 2-oxoglutarate, succinate, malate or citrate | Acidaminococcus fermentans | ? | - |
? | |
additional information | not involved in the dehydration of (R)-2-hydroxyglutarate to glutaconate | Acidaminococcus fermentans | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
octamer | alpha4beta4, 4 * 32000 + 4 * 34000, SDS-PAGE | Acidaminococcus fermentans |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | [Clostridium] sporosphaeroides |
25 | - |
assay at | [Clostridium] symbiosum |
25 | - |
assay at | Acidaminococcus fermentans |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Acidaminococcus fermentans |
7 | - |
optimum, glutaconyl-CoA + acetate | Acidaminococcus fermentans |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.6 | 7.4 | about 70% of maximal activity at pH 5.6 and 7.4 | Acidaminococcus fermentans |
8.5 | - |
about 10% of maximal activity at pH 8.5 | Acidaminococcus fermentans |