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Literature summary for 2.8.4.1 extracted from

  • Goubeau, M.; Schreiner, G.; Thauer, R.K.
    Purified methyl-coenzyme-M reductase is activated when the enzyme-bound coenzyme F-430 is reduced to the nickel(I) oxidation state by titanium(III) citrate (1997), Eur. J. Biochem., 243, 110-114.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
Ti(III) citrate conversion from the ox1 to the red1 state, maximal activation after 30 min, 20 mM Ti(III) at pH 9.0 and room temperature or pH 7.0 and 60°C, optimal pH for activation of isoenzyme II is 10.0, enzyme is activated when the enzyme bound coenzyme F-430 is reduced to the Ni(I) state Methanothermobacter thermautotrophicus

Metals/Ions

Metals/Ions Comment Organism Structure
Ni(2+)
-
Methanothermobacter thermautotrophicus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
300000
-
-
Methanothermobacter thermautotrophicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
methyl coenzyme M + coenzyme B Methanothermobacter thermautotrophicus methanogenesis methane + CoM-S-S-CoB
-
?
methyl coenzyme M + coenzyme B Methanothermobacter thermautotrophicus Marburg / DSM 2133 methanogenesis methane + CoM-S-S-CoB
-
?

Organism

Organism UniProt Comment Textmining
Methanothermobacter thermautotrophicus
-
-
-
Methanothermobacter thermautotrophicus Marburg / DSM 2133
-
-
-

Purification (Commentary)

Purification (Comment) Organism
4°C, strictly anaerobic conditions, fractional ammonium-sulfate precipitation Methanothermobacter thermautotrophicus
copurification of isoenzymes, separation of isoenzymes by anion-exchange chromatography Methanothermobacter thermautotrophicus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
activity measured at 92% N2, 8% H2 Methanothermobacter thermautotrophicus
100
-
-
Methanothermobacter thermautotrophicus

Storage Stability

Storage Stability Organism
4°C, pH 7.0, 4 h, 50% loss of activity of red1 form Methanothermobacter thermautotrophicus
storage in frozen stage not possible, thawing leads to a rapid loss of activity Methanothermobacter thermautotrophicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
methyl coenzyme M + coenzyme B methanogenesis Methanothermobacter thermautotrophicus methane + CoM-S-S-CoB
-
?
methyl coenzyme M + coenzyme B methanogenesis Methanothermobacter thermautotrophicus Marburg / DSM 2133 methane + CoM-S-S-CoB
-
?

Subunits

Subunits Comment Organism
hexamer alpha2,beta2,gamma2 Methanothermobacter thermautotrophicus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
65
-
assay at Methanothermobacter thermautotrophicus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
pH above 9.0 isoenzyme I is slowly denatured Methanothermobacter thermautotrophicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7 7.2 assay at Methanothermobacter thermautotrophicus

pH Stability

pH Stability pH Stability Maximum Comment Organism
9
-
above this pH value and 60°C isoenzyme I is slowly denatured Methanothermobacter thermautotrophicus

Cofactor

Cofactor Comment Organism Structure
F-430 2 mol nickel porphinoid/mol enzyme, prosthetic group Methanothermobacter thermautotrophicus