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Literature summary for 2.8.4.1 extracted from
- Purification and some properties of the methyl-CoM reductase of Methanothrix soehngenii (1990), FEMS Microbiol. Lett., 66, 183-186.
No PubMed abstract available
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| Ti(III) citrate | prevents enzyme inactivation by oxygen | Methanothrix soehngenii |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.023 | - |
coenzyme B | - |
Methanothrix soehngenii | |
| 2 | - |
methyl-coenzyme M | - |
Methanothrix soehngenii | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 30400 | - |
1 * 63900, 1 * 41700, 1 * 30400, SDS-PAGE | Methanothrix soehngenii |
| 41700 | - |
1 * 63900, 1 * 41700, 1 * 30400, SDS-PAGE | Methanothrix soehngenii |
| 63900 | - |
1 * 63900, 1 * 41700, 1 * 30400, SDS-PAGE | Methanothrix soehngenii |
| 280000 | - |
gel filtration | Methanothrix soehngenii |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Methanothrix soehngenii | - |
DSM 2139 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Q-Sepharose, Phenyl-Superose HR 5/5, Superose 6 HR 10/30 column | Methanothrix soehngenii |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.051 | - |
- |
Methanothrix soehngenii |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, stored under N2 atmosphere, 50 mM anaerobic Tris-HCl, pH 7.6, 150 mM NaCl, 1 mM Ti(III) citrate | Methanothrix soehngenii |
| 4°C | Methanothrix soehngenii |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| methyl-coenzyme M + coenzyme B | - |
Methanothrix soehngenii | methane + CoM-S-S-CoB | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| trimer | 1 * 63900, 1 * 41700, 1 * 30400, SDS-PAGE | Methanothrix soehngenii |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Methanothrix soehngenii |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
60 nmol of methane are formed per minute per mg protein | Methanothrix soehngenii |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| F-430 | contains two tightly bound molecules of coenzyme F-430 | Methanothrix soehngenii | |
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