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Literature summary for 2.8.4.1 extracted from

  • Gunsalus, R.P.; Wolfe, R.S.
    Methyl coenzyme M reductase from Methanobacterium thermoautotrophicum. Resolution and properties of the components (1980), J. Biol. Chem., 255, 1891-1895.
    View publication on PubMed
Search for more literature for 2.8.4.1

Activating Compound

Activating Compound Comment Organism Structure
additional information omission of the component A, B or C, as well as methyl coenzyme M, H2, Mg2+ or ATP leads to complete loss of activity and methane biosynthesis Methanothermobacter thermautotrophicus

Inhibitors

Inhibitors Comment Organism Structure
CO little blocking effect on the hydrogenase function of component A Methanothermobacter thermautotrophicus
O2 total loss of activity of hydrogenase activity of component A Methanothermobacter thermautotrophicus

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
 Methanothermobacter thermautotrophicus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
additional information
-
-
 Methanothermobacter thermautotrophicus

Organism

Organism UniProt Comment Textmining
Methanobacterium bryantii
-
 M.o.H. and M.o.H.G.
-
Methanobacterium formicicum
-
-
-
Methanosarcina barkeri
-
-
-
Methanospirillum hungatei
-
-
-
Methanothermobacter thermautotrophicus
-
-
-
no activity in Methanobrevibacter ruminantium
-
-
-
no activity in Methanobrevibacter ruminantium M-1
-
-
-

Oxidation Stability

Oxidation Stability Organism
oxygen causes irreversible loss of methane forming activity, component C is oxygen-stable Methanothermobacter thermautotrophicus

Purification (Commentary)

Purification (Comment) Organism
anaerobic conditions, ion-exchange column, gel-filtration Methanothermobacter thermautotrophicus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.00046
-
-
 Methanosarcina barkeri
0.000535
-
-
 Methanobacterium bryantii
0.00054
-
-
 Methanothermobacter thermautotrophicus
0.000935
-
-
 Methanobacterium formicicum
0.00094
-
-
 Methanobacterium bryantii
0.001135
-
-
 Methanospirillum hungatei

Storage Stability

Storage Stability Organism
ice bath temperature, hydrogenase stable 3-4 weeks Methanothermobacter thermautotrophicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
methyl-coenzyme M + coenzyme B in presence of H2 as source of electrons, requires Mg2+ and catalytic ATP Methanothermobacter thermautotrophicus methane + CoM-S-S-CoB
-
 ?
additional information ability of the hydrogenase to reduce a number of artificial and naturally occurring electron acceptors is examined Methanothermobacter thermautotrophicus ?
-
 ?

Subunits

Subunits Comment Organism
More three components: A 500000 Da hydrogenase activity for reduction of viologen dyes, coenzyme F-420 or flavins, B 1000 Da oxygen labile and C 130000 Da, gel-filtration Methanothermobacter thermautotrophicus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
 assay Methanothermobacter thermautotrophicus
60
-
 for hydrogenase of component A Methanothermobacter thermautotrophicus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
95
-
 10 min under anaerobic conditions, heat-labile components A and C lose activity Methanothermobacter thermautotrophicus
121
-
 1-2 h, heat-stable component B, no loss of activity Methanothermobacter thermautotrophicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
 assay at Methanothermobacter thermautotrophicus
7
-
 for hydrogenase of component A Methanothermobacter thermautotrophicus