Cloned (Comment) | Organism |
---|---|
- |
Saccharolobus shibatae |
overexpression in Escherichia coli as a soluble catalytically active protein | Saccharolobus shibatae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
HgCl2 | 1 mM, 11% residual activity; 1 mM, 89% inhibition | Saccharolobus shibatae | |
additional information | not inhibitory: EDTA, CaCl2, MgCl2, CuCl2, CoCl2, FeCl2, MnCl2, N-bromosuccinimide, diethylpyrocarbonate | Saccharolobus shibatae | |
phenylmethylsulfonyl fluoride | 1 mM, 4% inhibition; 1 mM, 4% residual activity | Saccharolobus shibatae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.01 | - |
p-nitrophenyl butanoate | - |
Saccharolobus shibatae | |
0.01 | - |
4-nitrophenyl butyrate | pH 7.2, 37°C | Saccharolobus shibatae | |
0.013 | - |
p-nitrophenyl acetate | - |
Saccharolobus shibatae | |
0.013 | - |
4-nitrophenyl acetate | pH 7.2, 37°C | Saccharolobus shibatae | |
0.039 | - |
p-nitrophenyl valerate | - |
Saccharolobus shibatae | |
0.039 | - |
4-nitrophenyl pentanoate | pH 7.2, 37°C | Saccharolobus shibatae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
33000 | - |
and drimer, 3 *33000, calculated, 3 * 33000, SDS-PAGE | Saccharolobus shibatae |
33000 | - |
and trimer, 2 *33000, calculated, 2 * 33000, SDS-PAGE | Saccharolobus shibatae |
33000 | - |
2 * 33000, enzyme exists as a mixture of dimeric and trimeric forms, SDS-PAGE | Saccharolobus shibatae |
33000 | - |
3 * 33000, enzyme exists as a mixture of dimeric and trimeric forms, SDS-PAGE | Saccharolobus shibatae |
64000 | - |
dimer, gel filtration | Saccharolobus shibatae |
90000 | - |
trimer, gel filtration | Saccharolobus shibatae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharolobus shibatae | - |
strain DSM5389, expression in Escherichia coli | - |
Saccharolobus shibatae | - |
DSM5389 | - |
Saccharolobus shibatae DSM 5389 | - |
strain DSM5389, expression in Escherichia coli | - |
Saccharolobus shibatae DSM 5389 | - |
DSM5389 | - |
Purification (Comment) | Organism |
---|---|
recombinant enzyme | Saccharolobus shibatae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl acetate + H2O | 15% of the activity with 4-nitrophenyl butyrate | Saccharolobus shibatae | 4-nitrophenol + acetate | - |
? | |
4-nitrophenyl acetate + H2O | 15% of the activity with 4-nitrophenyl butyrate | Saccharolobus shibatae DSM 5389 | 4-nitrophenol + acetate | - |
? | |
4-nitrophenyl butyrate + H2O | - |
Saccharolobus shibatae | 4-nitrophenol + butyrate | - |
? | |
4-nitrophenyl butyrate + H2O | - |
Saccharolobus shibatae DSM 5389 | 4-nitrophenol + butyrate | - |
? | |
4-nitrophenyl palmitate + H2O | - |
Saccharolobus shibatae | 4-nitrophenol + palmitate | - |
? | |
4-nitrophenyl pentanoate + H2O | 79% of the activity with 4-nitrophenyl butyrate | Saccharolobus shibatae | 4-nitrophenol + pentanoate | - |
? | |
additional information | no substrate: tributyrin, triolein | Saccharolobus shibatae | ? | - |
? | |
additional information | no hydrolysis of tributyrin and triolein | Saccharolobus shibatae | ? | - |
? | |
additional information | no substrate: tributyrin, triolein | Saccharolobus shibatae DSM 5389 | ? | - |
? | |
additional information | no hydrolysis of tributyrin and triolein | Saccharolobus shibatae DSM 5389 | ? | - |
? | |
p-nitrophenyl acetate + H2O | hydrolysis proceeds in linear manner, 15% of the reaction rate with p-nitrophenyl butyrate | Saccharolobus shibatae | p-nitrophenol + acetate | - |
? | |
p-nitrophenyl acetate + H2O | hydrolysis proceeds in linear manner, 15% of the reaction rate with p-nitrophenyl butyrate | Saccharolobus shibatae DSM 5389 | p-nitrophenol + acetate | - |
? | |
p-nitrophenyl butanoate + H2O | - |
Saccharolobus shibatae | p-nitrophenol + butanoate | - |
? | |
p-nitrophenyl pentanoate + H2O | and substrates with longer acyl chains, hydrolysis proceeds in biophasic manner | Saccharolobus shibatae | p-nitrophenol + pentanoate | - |
? | |
p-nitrophenyl valerate + H2O | 79% of the reaction rate with p-nitrophenyl butyrate | Saccharolobus shibatae | p-nitrophenol + valeric acid | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | and trimer, 2 *33000, calculated, 2 * 33000, SDS-PAGE | Saccharolobus shibatae |
dimer | 2 * 33000, enzyme exists as a mixture of dimeric and trimeric forms, SDS-PAGE | Saccharolobus shibatae |
trimer | and drimer, 3 *33000, calculated, 3 * 33000, SDS-PAGE | Saccharolobus shibatae |
trimer | 3 * 33000, enzyme exists as a mixture of dimeric and trimeric forms, SDS-PAGE | Saccharolobus shibatae |
Synonyms | Comment | Organism |
---|---|---|
SshEstI esterase | - |
Saccharolobus shibatae |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
60 min, pH 7.0, full activity | Saccharolobus shibatae |
90 | - |
30 min, pH 7.0, more than 70% residual activity | Saccharolobus shibatae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | 8 | - |
Saccharolobus shibatae |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
2.5 | - |
30 min, 60% residual activity | Saccharolobus shibatae |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
2.5 | - |
30 min, 60% residual activity | Saccharolobus shibatae |
2.5 | - |
60°C, 30 min, enzyme retains 60% of its initial activity | Saccharolobus shibatae |
6 | 8 | - |
Saccharolobus shibatae |