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Literature summary for 3.1.1.1 extracted from

  • Ileperuma, N.R.; Marshall, S.D.; Squire, C.J.; Baker, H.M.; Oakeshott, J.G.; Russell, R.J.; Plummer, K.M.; Newcomb, R.D.; Baker, E.N.
    High-resolution crystal structure of plant carboxylesterase AeCXE1, from Actinidia eriantha, and its complex with a high-affinity inhibitor paraoxon (2007), Biochemistry, 46, 1851-1859.
    View publication on PubMed
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Crystallization (Commentary)

Crystallization (Comment) Organism
free enzyme and in complex with inhibitor paraoxon. Enzyme is a member of the alpha/beta hydrolase superfamily. Conserved catalytic triad residues are S169, D276, and H306, in the native structure, S169 has a three-carbon acyl adduct bound. Inhibitor paraoxon binds covalently to the catalytic serine resiude Actinidia eriantha

Inhibitors

Inhibitors Comment Organism Structure
dimethylchlorophosphate
-
 Actinidia eriantha
paraoxon binds covalently to the catalytic serine residue Actinidia eriantha

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.000024
-
 4-methylumbelliferyl palmitate pH 8.0, 25°C Actinidia eriantha
0.000117
-
 4-methylumbelliferyl laurate pH 8.0, 25°C Actinidia eriantha
0.00634
-
 4-methylumbelliferyl octanoate pH 8.0, 25°C Actinidia eriantha
0.0167
-
 4-methylumbelliferyl butanoate pH 8.0, 25°C Actinidia eriantha
0.0191
-
 4-methylumbelliferyl heptanoate pH 8.0, 25°C Actinidia eriantha
0.0333
-
 4-yethylumbelliferyl acetate pH 8.0, 25°C Actinidia eriantha

Organism

Organism UniProt Comment Textmining
Actinidia eriantha Q0ZPV7 isoform CXE1
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-methylumbelliferyl acetate + H2O
-
 Actinidia eriantha 4-methylumbelliferol + acetate
-
 ?
4-methylumbelliferyl butyrate + H2O
-
 Actinidia eriantha 4-methylumbelliferol + butyrate
-
 ?
4-methylumbelliferyl heptanoate + H2O
-
 Actinidia eriantha 4-methylumbelliferol + heptanoate
-
 ?
4-methylumbelliferyl laurate + H2O
-
 Actinidia eriantha 4-methylumbelliferol + laurate
-
 ?
4-methylumbelliferyl octanoate + H2O
-
 Actinidia eriantha 4-methylumbelliferol + octanoate
-
 ?
4-methylumbelliferyl palmitate + H2O
-
 Actinidia eriantha 4-methylumbelliferol + palmitate
-
 ?
additional information enzyme hydrolyzes a range of carboxylester substrates with acyl groups ranging from C2 to C16, with a preference for butyryl moieties Actinidia eriantha ?
-
 ?

Synonyms

Synonyms Comment Organism
CXE
-
 Actinidia eriantha

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0018
-
 4-methylumbelliferyl palmitate pH 8.0, 25°C Actinidia eriantha
0.01
-
 4-methylumbelliferyl laurate pH 8.0, 25°C Actinidia eriantha
0.162
-
 4-methylumbelliferyl octanoate pH 8.0, 25°C Actinidia eriantha
1.61
-
 4-yethylumbelliferyl acetate pH 8.0, 25°C Actinidia eriantha
2
-
 4-methylumbelliferyl heptanoate pH 8.0, 25°C Actinidia eriantha
10.1
-
 4-methylumbelliferyl butanoate pH 8.0, 25°C Actinidia eriantha

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.0011
-
 pH 8.0, 25°C Actinidia eriantha paraoxon
0.0092
-
 pH 8.0, 25°C Actinidia eriantha dimethylchlorophosphate