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Literature summary for 3.1.1.1 extracted from

  • Streit, T.M.; Borazjani, A.; Lentz, S.E.; Wierdl, M.; Potter, P.M.; Gwaltney, S.R.; Ross, M.K.
    Evaluation of the side door in carboxylesterase-mediated catalysis and inhibition (2008), Biol. Chem., 389, 149-162.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Bacillus subtilis

Protein Variants

Protein Variants Comment Organism
L362A more sensitive to inhibition by organophosphates than wild-type Bacillus subtilis
L362D more sensitive to inhibition by organophosphates than wild-type Bacillus subtilis
L362E similar to mutant L362D, some decrease in kcat and Km value compared to wild-type Bacillus subtilis
L362R 22fold decrease in ratio kcat/Km value, mutant is less sensitive to organophosphates Bacillus subtilis

Inhibitors

Inhibitors Comment Organism Structure
methyl paraoxon
-
Bacillus subtilis
paraoxon
-
Bacillus subtilis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.01
-
trans-permethrin mutant L362R, 37°C, pH 7.4 Bacillus subtilis
0.013
-
trans-permethrin mutant L362A, 37°C, pH 7.4 Bacillus subtilis
0.014
-
trans-permethrin mutant L362D, 37°C, pH 7.4 Bacillus subtilis
0.014
-
trans-permethrin wild-type, 37°C, pH 7.4 Bacillus subtilis
0.1
-
4-nitrophenyl valerate mutant L362D, 37°C, pH 7.4 Bacillus subtilis
0.15
-
4-nitrophenyl valerate wild-type, 37°C, pH 7.4 Bacillus subtilis
0.23
-
4-yethylumbelliferyl acetate mutant L362D, 37°C, pH 7.4 Bacillus subtilis
0.26
-
4-nitrophenyl valerate mutant L362A, 37°C, pH 7.4 Bacillus subtilis
0.29
-
4-yethylumbelliferyl acetate wild-type, 37°C, pH 7.4 Bacillus subtilis
0.36
-
4-nitrophenyl valerate mutant L362R, 37°C, pH 7.4 Bacillus subtilis
0.43
-
4-yethylumbelliferyl acetate mutant L362A, 37°C, pH 7.4 Bacillus subtilis
0.57
-
4-yethylumbelliferyl acetate mutant L362R, 37°C, pH 7.4 Bacillus subtilis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
60000
-
PAGE Bacillus subtilis

Organism

Organism UniProt Comment Textmining
Bacillus subtilis
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-methylumbelliferyl acetate + H2O
-
Bacillus subtilis 4-methylumbelliferone + acetate
-
?
4-nitrophenyl valerate + H2O
-
Bacillus subtilis 4-nitrophenol + valerate
-
?
trans-permethrin + H2O
-
Bacillus subtilis (1S,3R)-3-(2,2-dichlorovinyl)-2,2-dimethylcyclopropanecarboxylic acid + (3-phenoxyphenyl)methanol
-
?

Subunits

Subunits Comment Organism
monomer 1 * 60000, SDS-PAGE and MALDI-TOF Bacillus subtilis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.5
-
trans-permethrin mutant L362R, 37°C, pH 7.4 Bacillus subtilis
2.1
-
trans-permethrin mutant L362A, 37°C, pH 7.4 Bacillus subtilis
2.2
-
trans-permethrin mutant L362E, 37°C, pH 7.4 Bacillus subtilis
2.9
-
trans-permethrin wild-type, 37°C, pH 7.4 Bacillus subtilis
57
-
4-yethylumbelliferyl acetate mutant L362R, 37°C, pH 7.4 Bacillus subtilis
69
-
4-nitrophenyl valerate mutant L362R, 37°C, pH 7.4 Bacillus subtilis
110
-
4-yethylumbelliferyl acetate mutant L362E, 37°C, pH 7.4 Bacillus subtilis
140
-
4-yethylumbelliferyl acetate wild-type, 37°C, pH 7.4 Bacillus subtilis
150
-
4-yethylumbelliferyl acetate mutant L362A, 37°C, pH 7.4 Bacillus subtilis
270
-
4-nitrophenyl valerate mutant L362E, 37°C, pH 7.4 Bacillus subtilis
300
-
4-nitrophenyl valerate mutant L362A, 37°C, pH 7.4 Bacillus subtilis
580
-
4-nitrophenyl valerate wild-type, 37°C, pH 7.4 Bacillus subtilis

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.0000008
-
mutant L362D, 37°C, pH 7.4 Bacillus subtilis paraoxon
0.000001
-
mutant L362A, 37°C, pH 7.4 Bacillus subtilis paraoxon
0.0000013
-
wild-type, 37°C, pH 7.4 Bacillus subtilis paraoxon
0.0000031
-
mutant L362R, 37°C, pH 7.4 Bacillus subtilis paraoxon
0.000018
-
mutant L362A, 37°C, pH 7.4 Bacillus subtilis methyl paraoxon
0.000024
-
mutant L362D, 37°C, pH 7.4 Bacillus subtilis methyl paraoxon
0.000081
-
wild-type, 37°C, pH 7.4 Bacillus subtilis methyl paraoxon
0.00027
-
mutant L362R, 37°C, pH 7.4 Bacillus subtilis methyl paraoxon