Application | Comment | Organism |
---|---|---|
synthesis | effective removal of t-butyl protecting group in organic synthesis | Bacillus subtilis |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Bacillus subtilis |
Protein Variants | Comment | Organism |
---|---|---|
G105A | 6fold increase in enantioselectivity against substrate (RS)-2-phenyl-3-butyn-2-yl acetate | Bacillus subtilis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
119 | - |
p-nitrophenyl acetate | pH 7.4 | Bacillus subtilis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | P37967 | isoform BS2, enzyme additionally shows amidase activity | - |
Storage Stability | Organism |
---|---|
-20°C, stable for at least 2 weeks | Bacillus subtilis |
-80°C, stable for at least 2 weeks | Bacillus subtilis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-phenyl-3-butyn-2-yl acetate | - |
Bacillus subtilis | 2-phenyl-3-butyn-2-ol + acetate | - |
? | |
3-methyl-1-pentyn-3-yl acetate + H2O | - |
Bacillus subtilis | 3-methyl-1-pentyn-3-ol + acetate | - |
? | |
linalyl acetate + H2O | - |
Bacillus subtilis | linalool + acetate | - |
? | |
additional information | enzyme activity against tertiary alcohol esters is about 1000fold lower compared with secondary and primary alcohol esters. The enantioselectivity towards esters of tertiary alcohols is very low | Bacillus subtilis | ? | - |
? | |
p-nitrophenol butanoate + H2O | - |
Bacillus subtilis | p-nitrophenol + butanoate | - |
? | |
p-nitrophenyl acetate + H2O | - |
Bacillus subtilis | p-nitrophenol + acetate | - |
? | |
t-butyl acetate + H2O | - |
Bacillus subtilis | t-butanol + acetate | - |
? | |
t-butyl-gamma-(t-butoxycarbonylamino)-butyrate + H2O | - |
Bacillus subtilis | ? | effective removal of t-butyl protecting group in organic synthesis | ? |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
active up to | Bacillus subtilis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
- |
40 | stable for at least 16 h | Bacillus subtilis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | 9 | - |
Bacillus subtilis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
10 | - |
completely inactive above | Bacillus subtilis |