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Literature summary for 3.1.1.1 extracted from

  • Park, H.; Kim, Y.; Kim, H.
    Expression and characterization of a new esterase cloned directly from Agrobacterium tumefaciens genome (2006), J. Microbiol. Biotechnol., 16, 145-148.
No PubMed abstract available
Search for more literature for 3.1.1.1

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli, His-tag Agrobacterium tumefaciens

Inhibitors

Inhibitors Comment Organism Structure
additional information not inhibitory: Ca2+, EDTA Agrobacterium tumefaciens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.761
-
 4-nitrophenyl acetate pH 7.0, 25°C Agrobacterium tumefaciens
0.86
-
 4-nitrophenyl butanoate pH 7.0, 25°C Agrobacterium tumefaciens
1.01
-
 1-naphthyl acetate pH 7.0, 25°C Agrobacterium tumefaciens
1.1
-
 1-naphthyl butanoate pH 7.0, 25°C Agrobacterium tumefaciens
2.01
-
 D-glucose pentaacetate pH 7.0, 25°C Agrobacterium tumefaciens
2.16
-
 D-xylose tetraacetate pH 7.0, 25°C Agrobacterium tumefaciens
3.02
-
 7-amino cephalosporanic acid pH 7.0, 25°C Agrobacterium tumefaciens

Organism

Organism UniProt Comment Textmining
Agrobacterium tumefaciens
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-naphthyl acetate + H2O
-
 Agrobacterium tumefaciens 1-naphthol + acetate
-
 ?
1-naphthyl butanoate + H2O
-
 Agrobacterium tumefaciens 1-naphthol + butanoate
-
 ?
4-nitrophenyl acetate + H2O
-
 Agrobacterium tumefaciens 4-nitrophenol + acetate
-
 ?
4-nitrophenyl butanoate + H2O
-
 Agrobacterium tumefaciens 4-nitrophenol + butanoate
-
 ?
7-amino cephalosporanic acid + H2O
-
 Agrobacterium tumefaciens ?
-
 ?
D-glucose pentaacetate + H2O
-
 Agrobacterium tumefaciens ? + acetate
-
 ?
D-xylose tetraacetate + H2O
-
 Agrobacterium tumefaciens ? + acetate
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
-
 Agrobacterium tumefaciens

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
 stable up to Agrobacterium tumefaciens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.22
-
 D-glucose pentaacetate pH 7.0, 25°C Agrobacterium tumefaciens
10.6
-
 4-nitrophenyl butanoate pH 7.0, 25°C Agrobacterium tumefaciens
11.6
-
 1-naphthyl acetate pH 7.0, 25°C Agrobacterium tumefaciens
11.8
-
 4-nitrophenyl acetate pH 7.0, 25°C Agrobacterium tumefaciens
11.8
-
 7-amino cephalosporanic acid pH 7.0, 25°C Agrobacterium tumefaciens
12.2
-
 D-xylose tetraacetate pH 7.0, 25°C Agrobacterium tumefaciens
18.7
-
 1-naphthyl butanoate pH 7.0, 25°C Agrobacterium tumefaciens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Agrobacterium tumefaciens

pH Stability

pH Stability pH Stability Maximum Comment Organism
5 9
-
 Agrobacterium tumefaciens