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Literature summary for 3.1.1.1 extracted from

  • Ay, F.; Karaoglu, H.; Inan, K.; Canakci, S.; Belduz, A.O.
    Cloning, purification and characterization of a thermostable carboxylesterase from Anoxybacillus sp. PDF1 (2011), Protein Expr. Purif., 80, 74-79.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
beta-mercaptoethanol 1% (v/v) activates carboxylesterase in a range of 136% and 244% after 0 and 30 min of incubation Anoxybacillus sp.
dithiothreitol 5 mM dithiothreitol increases the activity at 171% after the incubation of the enzyme for 30 min Anoxybacillus sp.

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Anoxybacillus sp.

Inhibitors

Inhibitors Comment Organism Structure
Co2+ about 42% residual activity at 5 mM Co2+ after 1 h of incubation Anoxybacillus sp.
EDTA 95% residual activity at 5 mM Anoxybacillus sp.
phenylmethylsulfonyl fluoride complete inhibition at 5 mM Anoxybacillus sp.
SDS 36% residual activity at 0.1% (w/v) after 30 min of incubation Anoxybacillus sp.
Zn2+ about 10% residual activity at 5 mM Zn2+ after 1 h of incubation Anoxybacillus sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.348
-
4-nitrophenyl butyrate apparent value, pH 8.0, 60°C Anoxybacillus sp.

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ 1 mM Ca2+ leads to some activation after 15 min Anoxybacillus sp.
K+ 1 mM K+ leads to some activation after 15 min Anoxybacillus sp.
Li+ 1 mM Li+ leads to some activation after 15 min Anoxybacillus sp.
additional information not influenced by Mg2+ Anoxybacillus sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
26000
-
x * 26000, SDS-PAGE Anoxybacillus sp.

Organic Solvent Stability

Organic Solvent Comment Organism
DMSO 97% residual activity at 1% (v/v) after 30 min of incubation Anoxybacillus sp.
Ethanol 92% residual activity at 1% (v/v) after 30 min of incubation Anoxybacillus sp.
isopropanol 99% residual activity at 1% (v/v) after 30 min of incubation Anoxybacillus sp.

Organism

Organism UniProt Comment Textmining
Anoxybacillus sp.
-
-
-
Anoxybacillus sp. PDF1
-
-
-

Purification (Commentary)

Purification (Comment) Organism
heat shock precipitation and Q-Sepharose column chromatography Anoxybacillus sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl butyrate + H2O high level of activity with 4-nitrophenyl butyrate (100%) Anoxybacillus sp. 4-nitrophenol + butyrate
-
?
4-nitrophenyl butyrate + H2O high level of activity with 4-nitrophenyl butyrate (100%) Anoxybacillus sp. PDF1 4-nitrophenol + butyrate
-
?
4-nitrophenyl laurate + H2O 37% activity compared to 4-nitrophenyl butyrate Anoxybacillus sp. 4-nitrophenol + laurate
-
?
4-nitrophenyl laurate + H2O 37% activity compared to 4-nitrophenyl butyrate Anoxybacillus sp. PDF1 4-nitrophenol + laurate
-
?
4-nitrophenyl palmitate + H2O 5% activity compared to 4-nitrophenyl butyrate Anoxybacillus sp. 4-nitrophenol + palmitate
-
?
4-nitrophenyl palmitate + H2O 5% activity compared to 4-nitrophenyl butyrate Anoxybacillus sp. PDF1 4-nitrophenol + palmitate
-
?
additional information carboxylesterase has serine residue in active site and -SH groups in specific sites which are required for its activity Anoxybacillus sp. ?
-
?
additional information carboxylesterase has serine residue in active site and -SH groups in specific sites which are required for its activity Anoxybacillus sp. PDF1 ?
-
?

Subunits

Subunits Comment Organism
? x * 26000, SDS-PAGE Anoxybacillus sp.

Synonyms

Synonyms Comment Organism
Carboxylic-ester hydrolase
-
Anoxybacillus sp.
EST
-
Anoxybacillus sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60 65
-
Anoxybacillus sp.

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
25 90 the enzymatic activity decreases significantly at temperatures below 25°C and above 90°C Anoxybacillus sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60 75 at 60 and 65°C, the enzyme is stable for half an hour; whereas incubation at 70°C for half an h leads to a 50% loss of activity. At 65°C, the stability of carboxylesterase decreases to 70% of the total. After 70°C, a sharp decline in the enzyme activity is observed Anoxybacillus sp.

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1500
-
4-nitrophenyl butyrate apparent value, pH 8.0, 60°C Anoxybacillus sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
Anoxybacillus sp.

pH Range

pH Minimum pH Maximum Comment Organism
5 10
-
Anoxybacillus sp.