Activating Compound | Comment | Organism | Structure |
---|---|---|---|
beta-mercaptoethanol | 1% (v/v) activates carboxylesterase in a range of 136% and 244% after 0 and 30 min of incubation | Anoxybacillus sp. | |
dithiothreitol | 5 mM dithiothreitol increases the activity at 171% after the incubation of the enzyme for 30 min | Anoxybacillus sp. |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Anoxybacillus sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Co2+ | about 42% residual activity at 5 mM Co2+ after 1 h of incubation | Anoxybacillus sp. | |
EDTA | 95% residual activity at 5 mM | Anoxybacillus sp. | |
phenylmethylsulfonyl fluoride | complete inhibition at 5 mM | Anoxybacillus sp. | |
SDS | 36% residual activity at 0.1% (w/v) after 30 min of incubation | Anoxybacillus sp. | |
Zn2+ | about 10% residual activity at 5 mM Zn2+ after 1 h of incubation | Anoxybacillus sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.348 | - |
4-nitrophenyl butyrate | apparent value, pH 8.0, 60°C | Anoxybacillus sp. |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 1 mM Ca2+ leads to some activation after 15 min | Anoxybacillus sp. | |
K+ | 1 mM K+ leads to some activation after 15 min | Anoxybacillus sp. | |
Li+ | 1 mM Li+ leads to some activation after 15 min | Anoxybacillus sp. | |
additional information | not influenced by Mg2+ | Anoxybacillus sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
26000 | - |
x * 26000, SDS-PAGE | Anoxybacillus sp. |
Organic Solvent | Comment | Organism |
---|---|---|
DMSO | 97% residual activity at 1% (v/v) after 30 min of incubation | Anoxybacillus sp. |
Ethanol | 92% residual activity at 1% (v/v) after 30 min of incubation | Anoxybacillus sp. |
isopropanol | 99% residual activity at 1% (v/v) after 30 min of incubation | Anoxybacillus sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Anoxybacillus sp. | - |
- |
- |
Anoxybacillus sp. PDF1 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
heat shock precipitation and Q-Sepharose column chromatography | Anoxybacillus sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl butyrate + H2O | high level of activity with 4-nitrophenyl butyrate (100%) | Anoxybacillus sp. | 4-nitrophenol + butyrate | - |
? | |
4-nitrophenyl butyrate + H2O | high level of activity with 4-nitrophenyl butyrate (100%) | Anoxybacillus sp. PDF1 | 4-nitrophenol + butyrate | - |
? | |
4-nitrophenyl laurate + H2O | 37% activity compared to 4-nitrophenyl butyrate | Anoxybacillus sp. | 4-nitrophenol + laurate | - |
? | |
4-nitrophenyl laurate + H2O | 37% activity compared to 4-nitrophenyl butyrate | Anoxybacillus sp. PDF1 | 4-nitrophenol + laurate | - |
? | |
4-nitrophenyl palmitate + H2O | 5% activity compared to 4-nitrophenyl butyrate | Anoxybacillus sp. | 4-nitrophenol + palmitate | - |
? | |
4-nitrophenyl palmitate + H2O | 5% activity compared to 4-nitrophenyl butyrate | Anoxybacillus sp. PDF1 | 4-nitrophenol + palmitate | - |
? | |
additional information | carboxylesterase has serine residue in active site and -SH groups in specific sites which are required for its activity | Anoxybacillus sp. | ? | - |
? | |
additional information | carboxylesterase has serine residue in active site and -SH groups in specific sites which are required for its activity | Anoxybacillus sp. PDF1 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 26000, SDS-PAGE | Anoxybacillus sp. |
Synonyms | Comment | Organism |
---|---|---|
Carboxylic-ester hydrolase | - |
Anoxybacillus sp. |
EST | - |
Anoxybacillus sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | 65 | - |
Anoxybacillus sp. |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | 90 | the enzymatic activity decreases significantly at temperatures below 25°C and above 90°C | Anoxybacillus sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | 75 | at 60 and 65°C, the enzyme is stable for half an hour; whereas incubation at 70°C for half an h leads to a 50% loss of activity. At 65°C, the stability of carboxylesterase decreases to 70% of the total. After 70°C, a sharp decline in the enzyme activity is observed | Anoxybacillus sp. |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1500 | - |
4-nitrophenyl butyrate | apparent value, pH 8.0, 60°C | Anoxybacillus sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Anoxybacillus sp. |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 10 | - |
Anoxybacillus sp. |