Application | Comment | Organism |
---|---|---|
analysis | wild-type enzyme and mutant enzyme N44S/S48V have the potential to be a biosensor for pesticide detection | Archaeoglobus fulgidus |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Archaeoglobus fulgidus |
Protein Variants | Comment | Organism |
---|---|---|
N44S | the sensitivity of the mutant enzyme for the large-sized inhibitor profenofos is 1.3fold of that observed for wild-type enzyme, but for the other inhibitors, the sensitivity decreases | Archaeoglobus fulgidus |
N44S/S48V | 1.5fold increase in esterase catalytic activity on 4-nitrophenyl dodecanoate. The sensitivity for the large-sized inhibitors chlorpyrifos, diazinon, triazophos, phoxim, and profenofos increases, which are 2.5-, 2.5-, 4.6-, 3.9-, and 3.1fold of that observed for wild-type AFEST, respectively | Archaeoglobus fulgidus |
S48V | the sensitivity of the mutant enzyme for the smallest inhibitor dichlorvos and medium-sized inhibitor phoxim is 1.6-, and 1.3fold of that observed for wild-type enzyme, respectively and has no obvious changes in the sensitivity towards paraoxon and large inhibitors (e.g., chlorpyrifos, triazophos, and profenofos). The IC50 of S48V for the large inhibitor diazinon is 1.9fold of that observed for wild-type enzyme | Archaeoglobus fulgidus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
diazinon | - |
Archaeoglobus fulgidus | |
dichlorvos | - |
Archaeoglobus fulgidus | |
paraoxon | - |
Archaeoglobus fulgidus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Archaeoglobus fulgidus | O28558 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Archaeoglobus fulgidus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl dodecanoate + H2O | - |
Archaeoglobus fulgidus | 4-nitrophenol + dodecanoate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
AF1716 | - |
Archaeoglobus fulgidus |
AFEST | - |
Archaeoglobus fulgidus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
assay at | Archaeoglobus fulgidus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
half-life for wild-type enzyme: 17 d, half-life for mutant enzyme N44S/S48V: 15 d | Archaeoglobus fulgidus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.1 | - |
assay at | Archaeoglobus fulgidus |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.0000059 | - |
pH 7.1, 50°C | Archaeoglobus fulgidus | paraoxon | |
0.0000508 | - |
pH 7.1, 50°C | Archaeoglobus fulgidus | dichlorvos | |
0.0075 | - |
pH 7.1, 50°C | Archaeoglobus fulgidus | diazinon |