Cloned (Comment) | Organism |
---|---|
- |
Pyrobaculum calidifontis |
General Stability | Organism |
---|---|
0.1% Triton X-100 exhibits a significant stabilizing effect without leading to a change in activity levels | Pyrobaculum calidifontis |
unstable at low concentrations, 10 - 100 nM. The half-life of the enzyme at a concentration of 60 nM is 5.2 h in 20 mM Tris-HCl (pH 8.0) at 4°C | Pyrobaculum calidifontis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
diethyldicarbonate | 2.0 mM, 98% inhibition | Pyrobaculum calidifontis | |
PMSF | 0.025, 94% inhibition | Pyrobaculum calidifontis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.044 | - |
4-nitrophenyl hexanoate | pH 7.1, 70°C | Pyrobaculum calidifontis | |
0.051 | - |
4-nitrophenyl octanoate | pH 7.1, 70°C | Pyrobaculum calidifontis | |
0.071 | - |
4-nitrophenyl pentanoate | pH 7.1, 70°C | Pyrobaculum calidifontis | |
0.164 | - |
4-nitrophenyl butanoate | pH 7.1, 70°C | Pyrobaculum calidifontis | |
0.348 | - |
4-nitrophenyl propanoate | pH 7.1, 70°C | Pyrobaculum calidifontis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
34000 | - |
3 * 34000, SDS-PAGE | Pyrobaculum calidifontis |
34354 | - |
3 * 34354, calculation from sequence | Pyrobaculum calidifontis |
98000 | - |
gel filtration | Pyrobaculum calidifontis |
Organic Solvent | Comment | Organism |
---|---|---|
2-propanol | 1 h, 80%, 6% loss of activity | Pyrobaculum calidifontis |
acetonitrile | 1 h, 80%, no loss of activity | Pyrobaculum calidifontis |
dimethyl sulfoxide | 1 h, 80%, 10% loss of activity | Pyrobaculum calidifontis |
dimethylformamide | 1 h, 80%, 4% loss of activity | Pyrobaculum calidifontis |
Ethanol | 1 h, 80%, no loss of activity | Pyrobaculum calidifontis |
Methanol | 1 h, 80%,% loss of activity | Pyrobaculum calidifontis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pyrobaculum calidifontis | Q8NKS0 | - |
- |
Pyrobaculum calidifontis VA1 | Q8NKS0 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Pyrobaculum calidifontis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
160 | - |
pH 7.1, 70°C, substrate: methyl acetate | Pyrobaculum calidifontis |
272 | - |
pH 7.1, 70°C, substrate: tert-butyl acetate | Pyrobaculum calidifontis |
313 | - |
pH 7.1, 70°C, substrate: ethyl acetate | Pyrobaculum calidifontis |
390 | - |
pH 7.1, 70°C, substrate: isopropyl acetate | Pyrobaculum calidifontis |
559 | - |
pH 7.1, 70°C, substrate: octyl acetate | Pyrobaculum calidifontis |
747 | - |
pH 7.1, 70°C, substrate: hexyl acetate | Pyrobaculum calidifontis |
805 | - |
pH 7.1, 70°C, substrate: propyl acetate | Pyrobaculum calidifontis |
876 | - |
pH 7.1, 70°C, substrate: sec-butyl acetate | Pyrobaculum calidifontis |
1160 | - |
pH 7.1, 70°C, substrate: butyl acetate | Pyrobaculum calidifontis |
1510 | - |
pH 7.1, 70°C, substrate: isobutyl acetate | Pyrobaculum calidifontis |
4050 | - |
pH 7.1, 70°C, substrate: 4-nitrophenyl hexanoate | Pyrobaculum calidifontis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl acetate + H2O | - |
Pyrobaculum calidifontis | 4-nitrophenol + acetate | - |
? | |
4-nitrophenyl acetate + H2O | - |
Pyrobaculum calidifontis VA1 | 4-nitrophenol + acetate | - |
? | |
4-nitrophenyl butanoate + H2O | - |
Pyrobaculum calidifontis | 4-nitrophenol + butanoate | - |
? | |
4-nitrophenyl hexanoate + H2O | - |
Pyrobaculum calidifontis | 4-nitrophenol + hexanoate | - |
? | |
4-nitrophenyl hexanoate + H2O | - |
Pyrobaculum calidifontis VA1 | 4-nitrophenol + hexanoate | - |
? | |
4-nitrophenyl octanoate + H2O | - |
Pyrobaculum calidifontis | 4-nitrophenol + octanoate | - |
? | |
4-nitrophenyl pentanoate + H2O | - |
Pyrobaculum calidifontis | 4-nitrophenol + pentanoate | - |
? | |
4-nitrophenyl pentanoate + H2O | - |
Pyrobaculum calidifontis VA1 | 4-nitrophenol + pentanoate | - |
? | |
4-nitrophenyl propanoate + H2O | - |
Pyrobaculum calidifontis | 4-nitrophenol + propanoate | - |
? | |
butyl acetate + H2O | - |
Pyrobaculum calidifontis | butanol + acetate | - |
? | |
ethyl acetate + H2O | - |
Pyrobaculum calidifontis | ethanol + acetate | - |
? | |
ethyl acetate + H2O | - |
Pyrobaculum calidifontis VA1 | ethanol + acetate | - |
? | |
isobutyl acetate + H2O | - |
Pyrobaculum calidifontis | isobutanol + acetate | - |
? | |
isopropyl acetate + H2O | - |
Pyrobaculum calidifontis | isopropanol + acetate | - |
? | |
methyl acetate + H2O | - |
Pyrobaculum calidifontis | methanol + acetate | - |
? | |
methyl acetate + H2O | - |
Pyrobaculum calidifontis VA1 | methanol + acetate | - |
? | |
octyl acetate + H2O | - |
Pyrobaculum calidifontis | octanol + acetate | - |
? | |
propyl acetate + H2O | - |
Pyrobaculum calidifontis | propanol + acetate | - |
? | |
sec-butyl acetate + H2O | - |
Pyrobaculum calidifontis | sec-butanol + acetate | - |
? | |
tert-butyl acetate + H2O | - |
Pyrobaculum calidifontis | tert-butanol + acetate | - |
? |
Subunits | Comment | Organism |
---|---|---|
trimer | 3 * 34000, SDS-PAGE | Pyrobaculum calidifontis |
trimer | 3 * 34354, calculation from sequence | Pyrobaculum calidifontis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
assay at | Pyrobaculum calidifontis |
90 | - |
- |
Pyrobaculum calidifontis |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 90 | activity at 30°C is 16% of the maximum activity (at 90°C) | Pyrobaculum calidifontis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
100 | - |
2 h, stable | Pyrobaculum calidifontis |
110 | - |
half-life: 56 min | Pyrobaculum calidifontis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1480 | - |
4-nitrophenyl propanoate | pH 7.1, 70°C | Pyrobaculum calidifontis | |
1600 | - |
4-nitrophenyl octanoate | pH 7.1, 70°C | Pyrobaculum calidifontis | |
1690 | - |
4-nitrophenyl butanoate | pH 7.1, 70°C | Pyrobaculum calidifontis | |
2070 | - |
4-nitrophenyl pentanoate | pH 7.1, 70°C | Pyrobaculum calidifontis | |
2620 | - |
4-nitrophenyl hexanoate | pH 7.1, 70°C | Pyrobaculum calidifontis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.1 | - |
assay at | Pyrobaculum calidifontis |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
4300 | - |
4-nitrophenyl propanoate | pH 7.1, 70°C | Pyrobaculum calidifontis | |
10000 | - |
4-nitrophenyl butanoate | pH 7.1, 70°C | Pyrobaculum calidifontis | |
29000 | - |
4-nitrophenyl pentanoate | pH 7.1, 70°C | Pyrobaculum calidifontis | |
31000 | - |
4-nitrophenyl octanoate | pH 7.1, 70°C | Pyrobaculum calidifontis | |
59000 | - |
4-nitrophenyl hexanoate | pH 7.1, 70°C | Pyrobaculum calidifontis |