Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | the addition of 1% (v/v) acetone and 1% (v/v) ethanol has little effect on enzyme activity | Alicyclobacillus tengchongensis |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Alicyclobacillus tengchongensis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ag+ | strong inhibition at 1 mM | Alicyclobacillus tengchongensis | |
Cu2+ | strong inhibition at 1 mM | Alicyclobacillus tengchongensis | |
Hg2+ | strong inhibition at 1 mM | Alicyclobacillus tengchongensis | |
SDS | strong inhibition at 1% (v/v) | Alicyclobacillus tengchongensis | |
Zn2+ | strong inhibition at 1 mM | Alicyclobacillus tengchongensis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1 | - |
2-naphthyl acetate | at pH 7.0 and 25°C | Alicyclobacillus tengchongensis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | activates at 1 mM | Alicyclobacillus tengchongensis | |
Pb2+ | activates at 1 mM | Alicyclobacillus tengchongensis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
57820 | - |
x * 57820, calculated from amino acid sequence | Alicyclobacillus tengchongensis |
58000 | - |
x * 58000, SDS-PAGE | Alicyclobacillus tengchongensis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
malathion + H2O | Alicyclobacillus tengchongensis | i.e. S-(1,2-dicarbethoxyethyl)-O,O-dimethyldithiophosphate | ? | - |
? | |
malathion + H2O | Alicyclobacillus tengchongensis D-1 | i.e. S-(1,2-dicarbethoxyethyl)-O,O-dimethyldithiophosphate | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Alicyclobacillus tengchongensis | S4TYB2 | - |
- |
Alicyclobacillus tengchongensis D-1 | S4TYB2 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni2+-NTA agarose column chromatography | Alicyclobacillus tengchongensis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-naphthyl acetate + H2O | - |
Alicyclobacillus tengchongensis | 2-naphthol + acetate | - |
? | |
2-naphthyl acetate + H2O | - |
Alicyclobacillus tengchongensis D-1 | 2-naphthol + acetate | - |
? | |
malathion + H2O | i.e. S-(1,2-dicarbethoxyethyl)-O,O-dimethyldithiophosphate | Alicyclobacillus tengchongensis | ? | - |
? | |
malathion + H2O | i.e. S-(1,2-dicarbethoxyethyl)-O,O-dimethyldithiophosphate | Alicyclobacillus tengchongensis D-1 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 58000, SDS-PAGE | Alicyclobacillus tengchongensis |
? | x * 57820, calculated from amino acid sequence | Alicyclobacillus tengchongensis |
Synonyms | Comment | Organism |
---|---|---|
D1CarE5 | - |
Alicyclobacillus tengchongensis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
- |
Alicyclobacillus tengchongensis |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 70 | more than 50% activity between 30 and 70°C | Alicyclobacillus tengchongensis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
the enzyme remains stable at 25°C for 50 min and loses about 25% and 65% of its activity after 50 min at 37°C and 60°C, respectively | Alicyclobacillus tengchongensis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Alicyclobacillus tengchongensis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.5 | 7.3 | more than 50% activity between pH 5.5 and 7.3 | Alicyclobacillus tengchongensis |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5.4 | 8.9 | the enzyme remains stable at pH 7.0 for 50 min and loses 20% and all of its activity after 50 min at pH 8.0 and 5.4, respectively | Alicyclobacillus tengchongensis |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Alicyclobacillus tengchongensis | calculated from amino acid sequence | - |
4.5 |