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Literature summary for 3.1.1.1 extracted from
- The development of bacterial carboxylesterase biological recognition elements for cocaine detection (2018), Mol. Biotechnol., 60, 601-607 .
Application
| Application | Comment | Organism |
|---|---|---|
| diagnostics | development of bacterial carboxylesterase biological recognition elements for cocaine detection | Bacillus subtilis subsp. subtilis |
| diagnostics | development of bacterial carboxylesterase biological recognition elements for cocaine detection | Bacillus licheniformis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus licheniformis | Q65MY7 | - |
- |
| Bacillus licheniformis ATCC 14580 | Q65MY7 | - |
- |
| Bacillus licheniformis DSM 13 | Q65MY7 | - |
- |
| Bacillus licheniformis Gibson 46 | Q65MY7 | - |
- |
| Bacillus licheniformis JCM 2505 | Q65MY7 | - |
- |
| Bacillus licheniformis NBRC 12200 | Q65MY7 | - |
- |
| Bacillus licheniformis NCIMB 9375 | Q65MY7 | - |
- |
| Bacillus licheniformis NRRL NRS-1264 | Q65MY7 | - |
- |
| Bacillus subtilis subsp. subtilis | P37967 | - |
- |
| Bacillus subtilis subsp. subtilis 168 | P37967 | - |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| cocaine + H2O | the enzyme mainly performs methyl ester hydrolysis. Spontaneous degradation of cocaine will start in a buffer with pH 7.0 at room temperature | Bacillus subtilis subsp. subtilis | benzoylecgonine + methanol | - |
? |  |
| cocaine + H2O | the enzyme mainly performs methyl ester hydrolysis. Spontaneous degradation of cocaine will start in a buffer with pH 7.0 at room temperature | Bacillus licheniformis | benzoylecgonine + methanol | - |
? | |
| cocaine + H2O | the enzyme mainly performs methyl ester hydrolysis. Spontaneous degradation of cocaine will start in a buffer with pH 7.0 at room temperature | Bacillus licheniformis NCIMB 9375 | benzoylecgonine + methanol | - |
? | |
| cocaine + H2O | the enzyme mainly performs methyl ester hydrolysis. Spontaneous degradation of cocaine will start in a buffer with pH 7.0 at room temperature | Bacillus licheniformis Gibson 46 | benzoylecgonine + methanol | - |
? | |
| cocaine + H2O | the enzyme mainly performs methyl ester hydrolysis. Spontaneous degradation of cocaine will start in a buffer with pH 7.0 at room temperature | Bacillus licheniformis JCM 2505 | benzoylecgonine + methanol | - |
? | |
| cocaine + H2O | the enzyme mainly performs methyl ester hydrolysis. Spontaneous degradation of cocaine will start in a buffer with pH 7.0 at room temperature | Bacillus licheniformis NRRL NRS-1264 | benzoylecgonine + methanol | - |
? | |
| cocaine + H2O | the enzyme mainly performs methyl ester hydrolysis. Spontaneous degradation of cocaine will start in a buffer with pH 7.0 at room temperature | Bacillus licheniformis NBRC 12200 | benzoylecgonine + methanol | - |
? | |
| cocaine + H2O | the enzyme mainly performs methyl ester hydrolysis. Spontaneous degradation of cocaine will start in a buffer with pH 7.0 at room temperature | Bacillus licheniformis ATCC 14580 | benzoylecgonine + methanol | - |
? | |
| cocaine + H2O | the enzyme mainly performs methyl ester hydrolysis. Spontaneous degradation of cocaine will start in a buffer with pH 7.0 at room temperature | Bacillus subtilis subsp. subtilis 168 | benzoylecgonine + methanol | - |
? | |
| cocaine + H2O | the enzyme mainly performs methyl ester hydrolysis. Spontaneous degradation of cocaine will start in a buffer with pH 7.0 at room temperature | Bacillus licheniformis DSM 13 | benzoylecgonine + methanol | - |
? | |
| additional information | for the benzoyl ester hydrolysis of cocaine cf. EC 3.1.1.84. Enzyme PnbA-1 resembles human hCE-1 enzyme in cocaine metabolism, hCE-1 enzyme hydrolyses the methyl ester of cocaine | Bacillus subtilis subsp. subtilis | ? | - |
? | |
| additional information | for the benzoyl ester hydrolysis of cocaine cf. EC 3.1.1.84. Enzyme PnbA-2 resembles human hCE-1 enzyme in cocaine metabolism, hCE-1 enzyme hydrolyses the methyl ester of cocaine | Bacillus licheniformis | ? | - |
? | |
| additional information | for the benzoyl ester hydrolysis of cocaine cf. EC 3.1.1.84. Enzyme PnbA-2 resembles human hCE-1 enzyme in cocaine metabolism, hCE-1 enzyme hydrolyses the methyl ester of cocaine | Bacillus licheniformis NCIMB 9375 | ? | - |
? | |
| additional information | for the benzoyl ester hydrolysis of cocaine cf. EC 3.1.1.84. Enzyme PnbA-2 resembles human hCE-1 enzyme in cocaine metabolism, hCE-1 enzyme hydrolyses the methyl ester of cocaine | Bacillus licheniformis Gibson 46 | ? | - |
? | |
| additional information | for the benzoyl ester hydrolysis of cocaine cf. EC 3.1.1.84. Enzyme PnbA-2 resembles human hCE-1 enzyme in cocaine metabolism, hCE-1 enzyme hydrolyses the methyl ester of cocaine | Bacillus licheniformis JCM 2505 | ? | - |
? | |
| additional information | for the benzoyl ester hydrolysis of cocaine cf. EC 3.1.1.84. Enzyme PnbA-2 resembles human hCE-1 enzyme in cocaine metabolism, hCE-1 enzyme hydrolyses the methyl ester of cocaine | Bacillus licheniformis NRRL NRS-1264 | ? | - |
? | |
| additional information | for the benzoyl ester hydrolysis of cocaine cf. EC 3.1.1.84. Enzyme PnbA-2 resembles human hCE-1 enzyme in cocaine metabolism, hCE-1 enzyme hydrolyses the methyl ester of cocaine | Bacillus licheniformis NBRC 12200 | ? | - |
? | |
| additional information | for the benzoyl ester hydrolysis of cocaine cf. EC 3.1.1.84. Enzyme PnbA-2 resembles human hCE-1 enzyme in cocaine metabolism, hCE-1 enzyme hydrolyses the methyl ester of cocaine | Bacillus licheniformis ATCC 14580 | ? | - |
? | |
| additional information | for the benzoyl ester hydrolysis of cocaine cf. EC 3.1.1.84. Enzyme PnbA-1 resembles human hCE-1 enzyme in cocaine metabolism, hCE-1 enzyme hydrolyses the methyl ester of cocaine | Bacillus subtilis subsp. subtilis 168 | ? | - |
? | |
| additional information | for the benzoyl ester hydrolysis of cocaine cf. EC 3.1.1.84. Enzyme PnbA-2 resembles human hCE-1 enzyme in cocaine metabolism, hCE-1 enzyme hydrolyses the methyl ester of cocaine | Bacillus licheniformis DSM 13 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 54000, SDS-PAGE | Bacillus subtilis subsp. subtilis |
| ? | x * 54000, SDS-PAGE | Bacillus licheniformis |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| pnbA | - |
Bacillus subtilis subsp. subtilis |
| pnbA | - |
Bacillus licheniformis |
| PnbA1 | - |
Bacillus subtilis subsp. subtilis |
| PnbA2 | - |
Bacillus licheniformis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
assay at | Bacillus subtilis subsp. subtilis |
| 40 | - |
assay at | Bacillus licheniformis |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | 45 | stable up to, activity drops sharply above 45°C | Bacillus subtilis subsp. subtilis |
| 25 | 55 | PnbA2 remains stable at temperatures ranging from 25-40°C, and stability of the enzyme decreases gradually above 40°C. PnbA2 activity gradually decreases with temperature increasing, the enzyme retain a part of its activity at 55°C | Bacillus licheniformis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | 9 | - |
Bacillus subtilis subsp. subtilis |
| 8 | 9 | - |
Bacillus licheniformis |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 11 | activity range | Bacillus subtilis subsp. subtilis |
| 6 | 11 | activity range | Bacillus licheniformis |
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