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Literature summary for 3.1.1.1 extracted from
- An antenna-biased carboxylesterase is specifically active to plant volatiles in Spodoptera exigua (2015), Pestic. Biochem. Physiol., 123, 93-100 .
Application
| Application | Comment | Organism |
|---|---|---|
| drug development | SexiCXE10 acts as a general odorant-degrading enzyme, showing the potential to be a target for developing behavioral antagonists and pesticides against Spodoptera exigua | Spodoptera exigua |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene SexiCXE10, DNA and amino acid sequence determination and analysis, phylogenetic tree, quantitative PCR expression analysis, recombinant expression of N-terminally His-tagged enzyme with Kozak sequences at N-terminus in High-Five cells of Trichoplusia ni using Autographa californica nucleopolyhedrovirus transfection | Spodoptera exigua |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.086 | - |
geranyl acetate | pH 7.4, 28°C | Spodoptera exigua |  |
| 2.423 | - |
(Z)-3-hexenyl caproate | pH 7.4, 28°C | Spodoptera exigua | |
| 3.135 | - |
(Z)-3-hexenyl butyrate | pH 7.4, 28°C | Spodoptera exigua | |
| 4.536 | - |
(E)-2-hexenyl butyrate | pH 7.4, 28°C | Spodoptera exigua | |
| 4.647 | - |
hexyl acetate | pH 7.4, 28°C | Spodoptera exigua | |
| 6.265 | - |
(Z)-3-hexenyl acetate | pH 7.4, 28°C | Spodoptera exigua | |
| 7.795 | - |
pentyl acetate | pH 7.4, 28°C | Spodoptera exigua | |
| 8.135 | - |
(E)-2-hexenyl acetate | pH 7.4, 28°C | Spodoptera exigua | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Spodoptera exigua | - |
- |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| antenna | from 6th instar larva heads, antenna-biased carboxylesterase | Spodoptera exigua | - |
| larva | 6th instar larva | Spodoptera exigua | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (E)-2-hexenyl acetate + H2O | - |
Spodoptera exigua | (E)-2-hexenol + acetate | - |
? | |
| (E)-2-hexenyl butyrate + H2O | - |
Spodoptera exigua | (E)-2-hexenol + butyrate | - |
? | |
| (Z)-3-hexenyl acetate + H2O | - |
Spodoptera exigua | (Z)-3-hexenol + acetate | - |
? | |
| (Z)-3-hexenyl butyrate + H2O | - |
Spodoptera exigua | (Z)-3-hexenol + butyrate | - |
? | |
| (Z)-3-hexenyl caproate + H2O | - |
Spodoptera exigua | (Z)-3-hexenol + caproate | - |
? | |
| geranyl acetate + H2O | - |
Spodoptera exigua | geraniol + acetate | - |
? | |
| heptyl acetate + H2O | lower activity | Spodoptera exigua | heptanol + acetate | - |
? | |
| hexyl acetate + H2O | - |
Spodoptera exigua | hexanol + acetate | - |
? | |
| additional information | no activity with nonyl acetate and unsaturated substrates with higher chain length, substrate specificity, overview | Spodoptera exigua | ? | - |
? | |
| octyl acetate + H2O | lower activity | Spodoptera exigua | octanol + acetate | - |
? | |
| pentyl acetate + H2O | - |
Spodoptera exigua | pentanol + acetate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 61380, sequence calculation | Spodoptera exigua |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| carboxyl/cholinesterase | - |
Spodoptera exigua |
| carboxylesterase | - |
Spodoptera exigua |
| CCE | - |
Spodoptera exigua |
| CXE | - |
Spodoptera exigua |
| general odorant degradation enzyme | - |
Spodoptera exigua |
| GODE | - |
Spodoptera exigua |
| ODE | - |
Spodoptera exigua |
| odorant degradation enzyme | - |
Spodoptera exigua |
| SexiCXE10 | - |
Spodoptera exigua |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 28 | - |
assay at | Spodoptera exigua |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.5 | - |
geranyl acetate | pH 7.4, 28°C | Spodoptera exigua | |
| 1.9 | - |
(Z)-3-hexenyl caproate | pH 7.4, 28°C | Spodoptera exigua | |
| 13.1 | - |
hexyl acetate | pH 7.4, 28°C | Spodoptera exigua | |
| 13.2 | - |
(Z)-3-hexenyl butyrate | pH 7.4, 28°C | Spodoptera exigua | |
| 21.9 | - |
(Z)-3-hexenyl acetate | pH 7.4, 28°C | Spodoptera exigua | |
| 22.5 | - |
(E)-2-hexenyl butyrate | pH 7.4, 28°C | Spodoptera exigua | |
| 26.1 | - |
(E)-2-hexenyl acetate | pH 7.4, 28°C | Spodoptera exigua | |
| 59 | - |
pentyl acetate | pH 7.4, 28°C | Spodoptera exigua | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 9 | - |
- |
Spodoptera exigua |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Spodoptera exigua | sequence calculation | - |
6.32 |
General Information
| General Information | Comment | Organism |
|---|---|---|
| evolution | the enzyme shows typical characteristics of the CCE gene family, including the catalytic triad comprised by Ser186, Glu345 and His463 | Spodoptera exigua |
| physiological function | SexiCXE10 acts as a general odorant-degrading enzyme and may play and important role in the detection of host plants. Female Spodoptera exigua responds significantly to (Z)-3-hexenyl acetate and other 4 volatiles with 7-10 C chain length, (E)-2-hexenyl acetate, heptyl acetate, octyl acetate and (Z)-3-hexenyl butyrate | Spodoptera exigua |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.45 | - |
geranyl acetate | pH 7.4, 28°C | Spodoptera exigua | |
| 0.78 | - |
(Z)-3-hexenyl caproate | pH 7.4, 28°C | Spodoptera exigua | |
| 2.82 | - |
hexyl acetate | pH 7.4, 28°C | Spodoptera exigua | |
| 3.21 | - |
(E)-2-hexenyl acetate | pH 7.4, 28°C | Spodoptera exigua | |
| 3.59 | - |
(Z)-3-hexenyl acetate | pH 7.4, 28°C | Spodoptera exigua | |
| 4.21 | - |
(Z)-3-hexenyl butyrate | pH 7.4, 28°C | Spodoptera exigua | |
| 4.96 | - |
(E)-2-hexenyl butyrate | pH 7.4, 28°C | Spodoptera exigua | |
| 7.57 | - |
pentyl acetate | pH 7.4, 28°C | Spodoptera exigua | |
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