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Literature summary for 3.1.1.11 extracted from

  • Dirix, C.; Duvetter, T.; Loey, A.V.; Hendrickx, M.; Heremans, K.
    The in situ observation of the temperature and pressure stability of recombinant Aspergillus aculeatus pectin methylesterase with Fourier transform IR spectroscopy reveals an unusual pressure stability of beta-helices (2005), Biochem. J., 392, 565-571.
    View publication on PubMedView publication on EuropePMC

Application

Application Comment Organism
food industry used for various applications in fruit processing e.g. texture improvement of fruit pieces, juice extraction, concentration and clarification of fruit juices Aspergillus aculeatus

General Stability

General Stability Organism
pressure increases stability towards thermal inativation Aspergillus aculeatus
very pressure stable, no inactivation up to 700 MPa Aspergillus aculeatus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
pectin + H2O Aspergillus aculeatus involved in the regulation of the cell wall rigidity, key role in process of fruit ripening methanol + pectate
-
?

Organism

Organism UniProt Comment Textmining
Aspergillus aculeatus
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
pectin + H2O apple pectin Aspergillus aculeatus methanol + pectate
-
?
pectin + H2O involved in the regulation of the cell wall rigidity, key role in process of fruit ripening Aspergillus aculeatus methanol + pectate
-
?

Synonyms

Synonyms Comment Organism
pectin methylesterase
-
Aspergillus aculeatus
PME
-
Aspergillus aculeatus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
43% loss of activity within 15 min at atmospheric pressure Aspergillus aculeatus
55
-
97% loss of activity within 15 min at atmospheric pressure, no inactivation at pressures above 200 MPa up to at least 700 MPa Aspergillus aculeatus
55
-
no inactivation at pressures above 400 MPa up to at least 700 MPa Aspergillus aculeatus
65
-
progressive loss of activity even at high pressure conditions Aspergillus aculeatus