Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli strain M15, the recombinant PME proteins (full-length and mature) do not show either PME or RIP activity | Arabidopsis thaliana |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
35000 | - |
SDS-PAGE | Arabidopsis thaliana |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | - |
ecotype Col-0 | - |
Purification (Comment) | Organism |
---|---|
Ni-NTA His-bind resin chromatography, DEAE-Sephadex gel filtration, CM-cellulose column chromatography, and Shephacryl SH-100 gel filtration | Arabidopsis thaliana |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
whole plant | - |
Arabidopsis thaliana | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
pectin + H2O | - |
Arabidopsis thaliana | methanol + pectate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
pectin methylesterase | - |
Arabidopsis thaliana |
PME | - |
Arabidopsis thaliana |
RIP | the RIP purified is identified as a mature form of pectin methylesterase, the purified native protein shows both pectin methylesterase and ribosome-inactivating protein activity | Arabidopsis thaliana |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Arabidopsis thaliana |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
9 | - |
no activity at pH 9 | Arabidopsis thaliana |