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Literature summary for 3.1.1.11 extracted from

  • Laratta, B.; De Masi, L.; Minasi, P.; Giovane, A.
    Pectin methylesterase in Citrus bergamia R.: purification, biochemical characterization and sequence of the exon related to the enzyme active site (2008), Food Chem., 110, 829-837.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
NaCl a 10% increase of activity is observed at 0.1 M NaCl in all the three isoforms, then the activity decreases almost linearly with salt concentration increase for the PME I, while PME II and PME III show little increase of activity when salt concentration reaches 0.35 M Citrus bergamia

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
42000
-
the three PME isoforms show the same molecular weight of 42000 Da, SDS-PAGE Citrus bergamia

Organism

Organism UniProt Comment Textmining
Citrus bergamia Q1PAH6 fragment
-

Purification (Commentary)

Purification (Comment) Organism
heparin-Sepharose column chromatography, S-sepharose column chromatography, and Superdex-75 gel filtration Citrus bergamia

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
Citrus bergamia
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1
-
crude homogenate Citrus bergamia
2253
-
isozyme PME I after purification Citrus bergamia
2297
-
isozyme PME II after purification Citrus bergamia
2333
-
isozyme PME III after purification Citrus bergamia

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
pectin + H2O
-
Citrus bergamia methanol + pectate
-
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Synonyms

Synonyms Comment Organism
pectin esterase
-
Citrus bergamia
pectin methylesterase
-
Citrus bergamia
PME I isozyme Citrus bergamia
PME II isozyme Citrus bergamia
PME III isozyme Citrus bergamia

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
65 80 PME I and PME III retain about the 80% of their activity after 4 min of incubation at 65°C, whereas PME II retains 60% of activity, PME I is more resistant at 80°C than the other PME isoforms, retaining about 9% of its activity after 30 s where PME II and PME III retain only 1% of their activity Citrus bergamia

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
isozyme PME II, at 0.1 M NaCl isozyme PME III shows two maxima at pH 7.0 and 9.0 Citrus bergamia
7.5
-
isozyme PME I Citrus bergamia
9
-
isozyme PME III, at 0.35 M NaCl only one maximum is observed at pH 9.0 Citrus bergamia

pH Range

pH Minimum pH Maximum Comment Organism
4.5 9.5
-
Citrus bergamia