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Literature summary for 3.1.1.11 extracted from

  • Dedeurwaerder, S.; Menu-Bouaouiche, L.; Mareck, A.; Lerouge, P.; Guerineau, F.
    Activity of an atypical Arabidopsis thaliana pectin methylesterase (2009), Planta, 229, 311-321.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli strain JM101, IPTG induction at 37°C instead of 25-28°C results in approximately 10times less PME activity in the extract Arabidopsis thaliana

Inhibitors

Inhibitors Comment Organism Structure
additional information a purified kiwi (Actinidia chinensis) pectin methylesterase inhibitor has no effect on the activity of the enzyme Arabidopsis thaliana
NaCl activity decreases in the presence of 0.1 M NaCl and is 4times lower in 0.5 M NaCl Arabidopsis thaliana

Metals/Ions

Metals/Ions Comment Organism Structure
additional information the enzyme does not require salt for activity Arabidopsis thaliana

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
37000
-
SDS-PAGE Arabidopsis thaliana

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation and Ni-NTA agarose column chromatography Arabidopsis thaliana

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.246
-
crude extract Arabidopsis thaliana
11.98
-
purified enzyme Arabidopsis thaliana

Storage Stability

Storage Stability Organism
-20°C, crude extract, several weeks, no significant loss of activity Arabidopsis thaliana

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
pectin + H2O
-
Arabidopsis thaliana methanol + pectate
-
?

Synonyms

Synonyms Comment Organism
pectin methylesterase
-
Arabidopsis thaliana
PME31
-
Arabidopsis thaliana

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30 55 incubation at 30°C has little but significant effect on enzyme activity, the incubation at 55°C abolishes over 95% of enzyme activity Arabidopsis thaliana

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8 8.5 in phosphate buffer Arabidopsis thaliana
9
-
around pH 9 in glycine buffer Arabidopsis thaliana