Cloned (Comment) | Organism |
---|---|
expressed in Pichia pastoris | Sphenophorus levis |
Crystallization (Comment) | Organism |
---|---|
sitting drop vapor diffusion method, using 25% (w/v) PEG 3350, 0.1 M citric acid pH 3.5 | Sphenophorus levis |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
50000 | - |
x * 50000, SDS-PAGE | Sphenophorus levis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
pectin + H2O | Sphenophorus levis | - |
methanol + pectate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sphenophorus levis | V9Q624 | sugar cane weevil | - |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography | Sphenophorus levis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
pectin + H2O | - |
Sphenophorus levis | methanol + pectate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 50000, SDS-PAGE | Sphenophorus levis |
Synonyms | Comment | Organism |
---|---|---|
pectin methylesterase | - |
Sphenophorus levis |
PME | - |
Sphenophorus levis |