Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli Top10F' cells | Citrus sinensis |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cell wall | - |
Citrus sinensis | 5618 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
NaCl | highest activity in the presence of 1 M NaCl. No enzyme activity (assayed at pH 7.0) is detected in salt-free water washes of pulp (measured at pH 3.8) | Citrus sinensis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
47900 | - |
x * 47900, one of two principal glycoisoforms, MALDI-TOF mass spectrometry | Citrus sinensis |
53000 | - |
x * 53000, one of two principal glycoisoforms, MALDI-TOF mass spectrometry | Citrus sinensis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
pectin + H2O | Citrus sinensis | - |
methanol + pectate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Citrus sinensis | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | the enzyme forms exhibits two principal glycoisoforms | Citrus sinensis |
Purification (Comment) | Organism |
---|---|
DEAE-Sepharose column chromatography and PME-IP affinity chromatography | Citrus sinensis |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
fruit | - |
Citrus sinensis | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
5.9 | - |
cell wall extract, at pH 7.0 and 30°C | Citrus sinensis |
963 | - |
after 163fold purification, at pH 7.0 and 30°C | Citrus sinensis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
pectin + H2O | - |
Citrus sinensis | methanol + pectate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 47900, one of two principal glycoisoforms, MALDI-TOF mass spectrometry | Citrus sinensis |
? | x * 53000, one of two principal glycoisoforms, MALDI-TOF mass spectrometry | Citrus sinensis |
Synonyms | Comment | Organism |
---|---|---|
pectin methylesterase | - |
Citrus sinensis |
PME | - |
Citrus sinensis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Citrus sinensis |