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Literature summary for 3.1.1.11 extracted from

  • Savary, B.J.; Vasu, P.; Cameron, R.G.; McCollum, T.G.; Nunez, A.
    Structural characterization of the thermally tolerant pectin methylesterase purified from Citrus sinensis fruit and its gene sequence (2013), J. Agric. Food Chem., 61, 12711-12719.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Top10F' cells Citrus sinensis

Localization

Localization Comment Organism GeneOntology No. Textmining
cell wall
-
Citrus sinensis 5618
-

Metals/Ions

Metals/Ions Comment Organism Structure
NaCl highest activity in the presence of 1 M NaCl. No enzyme activity (assayed at pH 7.0) is detected in salt-free water washes of pulp (measured at pH 3.8) Citrus sinensis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
47900
-
x * 47900, one of two principal glycoisoforms, MALDI-TOF mass spectrometry Citrus sinensis
53000
-
x * 53000, one of two principal glycoisoforms, MALDI-TOF mass spectrometry Citrus sinensis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
pectin + H2O Citrus sinensis
-
methanol + pectate
-
?

Organism

Organism UniProt Comment Textmining
Citrus sinensis
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein the enzyme forms exhibits two principal glycoisoforms Citrus sinensis

Purification (Commentary)

Purification (Comment) Organism
DEAE-Sepharose column chromatography and PME-IP affinity chromatography Citrus sinensis

Source Tissue

Source Tissue Comment Organism Textmining
fruit
-
Citrus sinensis
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
5.9
-
cell wall extract, at pH 7.0 and 30°C Citrus sinensis
963
-
after 163fold purification, at pH 7.0 and 30°C Citrus sinensis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
pectin + H2O
-
Citrus sinensis methanol + pectate
-
?

Subunits

Subunits Comment Organism
? x * 47900, one of two principal glycoisoforms, MALDI-TOF mass spectrometry Citrus sinensis
? x * 53000, one of two principal glycoisoforms, MALDI-TOF mass spectrometry Citrus sinensis

Synonyms

Synonyms Comment Organism
pectin methylesterase
-
Citrus sinensis
PME
-
Citrus sinensis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
Citrus sinensis