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Literature summary for 3.1.1.11 extracted from

  • L'Enfant, M.; Domon, J.M.; Rayon, C.; Desnos, T.; Ralet, M.C.; Bonnin, E.; Pelloux, J.; Pau-Roblot, C.
    Substrate specificity of plant and fungi pectin methylesterases identification of novel inhibitors of PMEs (2015), Int. J. Biol. Macromol., 81, 681-691 .
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
recombinant expression in Pichia pastoris strain GS115, the enzyme is secreted to the culture medium Botrytis cinerea
recombinant overexpression of His-tagged isozyme PME31 in Escherichia coli strain JM101 Arabidopsis thaliana

Inhibitors

Inhibitors Comment Organism Structure
(E)-1-(2-nitroethenyl)-4-(2-propenyloxy)-benzene
-
Arabidopsis thaliana
(E)-1-(2-nitroethenyl)-4-(2-propenyloxy)-benzene
-
Citrus sinensis
1-(3,4-dihydroxyphenyl)-2-([1-(1-naphthyl)-1H-tetrazol-5-yl]thio)ethanone
-
Arabidopsis thaliana
1-(3,4-dihydroxyphenyl)-2-([1-(1-naphthyl)-1H-tetrazol-5-yl]thio)ethanone
-
Citrus sinensis
1-(3,6-dichloro-9H-carbazoyl-9-yl)-3-morpholinopropan-2-ol
-
Arabidopsis thaliana
1-(3,6-dichloro-9H-carbazoyl-9-yl)-3-morpholinopropan-2-ol
-
Citrus sinensis
10-(hydroxymethylene)-9(10H)-phenanthrenone
-
Arabidopsis thaliana
10-(hydroxymethylene)-9(10H)-phenanthrenone
-
Citrus sinensis
2-(2-[4-(trifluoromethyl)phenyl]hydrazylidene)propanedinitrile
-
Arabidopsis thaliana
2-(2-[4-(trifluoromethyl)phenyl]hydrazylidene)propanedinitrile
-
Citrus sinensis
2-(3,4-dihydro-1(2H)-naphthalenylidene)hydrazinecarbothioamide
-
Arabidopsis thaliana
2-(3,4-dihydro-1(2H)-naphthalenylidene)hydrazinecarbothioamide
-
Citrus sinensis
2-(3-chloro-2-fluorophenyl)-2,3-dihydroisothiazol-3-one
-
Arabidopsis thaliana
2-(3-chloro-2-fluorophenyl)-2,3-dihydroisothiazol-3-one
-
Citrus sinensis
2-chloro-3-(2-methylphenoxy)-1,4-dihydro-naphthalene-1,4-dione
-
Arabidopsis thaliana
2-chloro-3-(2-methylphenoxy)-1,4-dihydro-naphthalene-1,4-dione
-
Citrus sinensis
2-methoxyacridin-9-amine
-
Arabidopsis thaliana
2-methoxyacridin-9-amine
-
Citrus sinensis
2-[(4-chlorophenyl)sulfonyl]-2,4-dihydro-4-(2-propen-1-yl)-5-propyl-3H-pyrazol-3-one
-
Arabidopsis thaliana
2-[(4-chlorophenyl)sulfonyl]-2,4-dihydro-4-(2-propen-1-yl)-5-propyl-3H-pyrazol-3-one
-
Citrus sinensis
3H-pyrazol-3-one
-
Arabidopsis thaliana
3H-pyrazol-3-one
-
Citrus sinensis
4-(3-hydroxy-3-methylbut-1-ynyl)benzaldehyde-2-phenylhydrazone
-
Arabidopsis thaliana
4-(3-hydroxy-3-methylbut-1-ynyl)benzaldehyde-2-phenylhydrazone
-
Citrus sinensis
4-[1-(fur-2-oyl)pyrazol-5-yl]-5-methyl-1-phenylpyrazole
-
Arabidopsis thaliana
4-[1-(fur-2-oyl)pyrazol-5-yl]-5-methyl-1-phenylpyrazole
-
Citrus sinensis
copper sulfate
-
Arabidopsis thaliana
copper sulfate only slight inhibition Citrus sinensis
hydrazine
-
Arabidopsis thaliana
hydrazine
-
Citrus sinensis
additional information analysis of inhibition of the enzyme by polyphenols such as catechins and tannic acid, overview Arabidopsis thaliana
additional information analysis of inhibition of the enzyme by polyphenols such as catechins and tannic acid, or polyphenon 60 mixture, overview. The fungal PME is not sensitive to inhibition, overview Botrytis cinerea
additional information analysis of inhibition of the enzyme by polyphenols such as catechins and tannic acid, overview Citrus sinensis
N'1-[2-(tert-butyl)-5-(trifluoromethyl)-pyrazolo[1,5-alpha]-pyrimidin-7-yl]-4-chlorobenzenohydrazide
-
Arabidopsis thaliana
N'1-[2-(tert-butyl)-5-(trifluoromethyl)-pyrazolo[1,5-alpha]-pyrimidin-7-yl]-4-chlorobenzenohydrazide
-
Citrus sinensis
N'6-[3,5-di-(trifluoromethyl)phenyl]-5-oxo-2,3-dihydro-5H-pyrimido-[2,1-b][1,3]thiazole-6-carbohydrazide
-
Arabidopsis thaliana
N'6-[3,5-di-(trifluoromethyl)phenyl]-5-oxo-2,3-dihydro-5H-pyrimido-[2,1-b][1,3]thiazole-6-carbohydrazide
-
Citrus sinensis
N-(1,4-dibenzoyl-5-phenyl-4,5-dihydro-1H-1,2,4-triazol-3-yl)-benzamide
-
Arabidopsis thaliana
N-(1,4-dibenzoyl-5-phenyl-4,5-dihydro-1H-1,2,4-triazol-3-yl)-benzamide
-
Citrus sinensis
N-(3,5-dichlorophenyl)-N'-[5-(2-furyl)-1H-pyrazol-3-yl]urea
-
Arabidopsis thaliana
N-(3,5-dichlorophenyl)-N'-[5-(2-furyl)-1H-pyrazol-3-yl]urea
-
Citrus sinensis
N-(4-chloro-2-nitrophenyl)-N'-phenylurea
-
Arabidopsis thaliana
N-(4-chloro-2-nitrophenyl)-N'-phenylurea
-
Citrus sinensis
N-(4-methyl-2-thienyl)-N'-[4-(trifluoromethyl)phenyl]-urea
-
Arabidopsis thaliana
N-(4-methyl-2-thienyl)-N'-[4-(trifluoromethyl)phenyl]-urea
-
Citrus sinensis
N-[(2-chlorobenzoyl)oxy]-2,1,3-benzoxadiazole-5-carboximidamide
-
Arabidopsis thaliana
N-[(2-chlorobenzoyl)oxy]-2,1,3-benzoxadiazole-5-carboximidamide
-
Citrus sinensis
N1-[3-(trifluoromethyl)phenyl]-3-(2-thienylthio)-propanamide
-
Arabidopsis thaliana
N1-[3-(trifluoromethyl)phenyl]-3-(2-thienylthio)-propanamide
-
Citrus sinensis
N4-(2-furylmethyl)-2-(2,3-dihydro-1,4-benzodioxin-2-yl)-1,3-thiazole-4-carboxamide
-
Arabidopsis thaliana
N4-(2-furylmethyl)-2-(2,3-dihydro-1,4-benzodioxin-2-yl)-1,3-thiazole-4-carboxamide
-
Citrus sinensis
phenylmercuric acetate
-
Arabidopsis thaliana
phenylmercuric acetate
-
Citrus sinensis
polyphenon 60 PP60, commercial inhibitor mixture that contains 60% catechin (which consists of 34% (-)-epigallocatechin-3-gallate, 16.7% (-)-epigallocatechin, 8.7% (-)-epicatechin-3-gallate, 7.3% (-)-epicatechin, 2.8% (-)-gallocatechin gallate, and 0.5% (-)-catechin gallate), tannic acid, or (-)-epigallocatechin-3-gallate Arabidopsis thaliana
polyphenon 60 PP60, commercial inhibitor mixture that contains 60% catechin (which consists of 34% (-)-epigallocatechin-3-gallate, 16.7% (-)-epigallocatechin, 8.7% (-)-epicatechin-3-gallate, 7.3% (-)-epicatechin, 2.8% (-)-gallocatechin gallate, and 0.5% (-)-catechin gallate), tannic acid, or (-)-epigallocatechin-3-gallate Citrus sinensis
Silver nitrate complete inhibition Arabidopsis thaliana
Silver nitrate complete inhibition Citrus sinensis
[5-(4-chlorophenyl)-3-thienyl]-(piperidino)-methanone
-
Arabidopsis thaliana
[5-(4-chlorophenyl)-3-thienyl]-(piperidino)-methanone
-
Citrus sinensis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
pectin + n H2O Citrus sinensis
-
n methanol + pectate
-
?
pectin + n H2O Arabidopsis thaliana
-
n methanol + pectate
-
?
pectin + n H2O Botrytis cinerea
-
n methanol + pectate
-
?
pectin + n H2O Botrytis cinerea B05.10
-
n methanol + pectate
-
?

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana Q9LVQ0
-
-
Botrytis cinerea A0A384J5R9 i.e. Botryotinia fuckeliana
-
Botrytis cinerea B05.10 A0A384J5R9 i.e. Botryotinia fuckeliana
-
Citrus sinensis O04886 PME1
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme from Pichia pastoris strain GS115 culture medium by dialysis and anion exchange chromatography Botrytis cinerea
recombinant His-tagged isozyme PME31 from Escherichia coli strain JM101 by nickel affinity chromatography Arabidopsis thaliana

Source Tissue

Source Tissue Comment Organism Textmining
commercial preparation
-
Citrus sinensis
-
fruit peel
-
Citrus sinensis
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
homogalacturonan + H2O different homogalacturonan substrates, best at pH 4.0-6.0 for enzyme BcPME Citrus sinensis ?
-
?
homogalacturonan + H2O different homogalacturonan substrates, best at pH 4.0-6.0 for enzyme BcPME Arabidopsis thaliana ?
-
?
homogalacturonan + H2O different homogalacturonan substrates, best at pH 4.0-6.0 for enzyme BcPME Botrytis cinerea ?
-
?
homogalacturonan + H2O different homogalacturonan substrates, best at pH 4.0-6.0 for enzyme BcPME Botrytis cinerea B05.10 ?
-
?
additional information substrate specificity, overview. Homogalacturonan substrates are HG96B20, HG96B39 HG96B56, HG96B69, HG96B82, HG96P14, HG96P36, HG96P56, HG96P64, and HG96P75. Spectrometric quantification Citrus sinensis ?
-
?
additional information substrate specificity, overview. Homogalacturonan substrates are HG96B20, HG96B39 HG96B56, HG96B69, HG96B82, HG96P14, HG96P36, HG96P56, HG96P64, and HG96P75. Spectrometric quantification Arabidopsis thaliana ?
-
?
additional information substrate specificity, overview. Homogalacturonan substrates are HG96B20, HG96B39 HG96B56, HG96B69, HG96B82, HG96P14, HG96P36, HG96P56, HG96P64, and HG96P75. Spectrometric quantification Botrytis cinerea ?
-
?
additional information substrate specificity, overview. Homogalacturonan substrates are HG96B20, HG96B39 HG96B56, HG96B69, HG96B82, HG96P14, HG96P36, HG96P56, HG96P64, and HG96P75. Spectrometric quantification Botrytis cinerea B05.10 ?
-
?
pectin + n H2O
-
Citrus sinensis n methanol + pectate
-
?
pectin + n H2O
-
Arabidopsis thaliana n methanol + pectate
-
?
pectin + n H2O
-
Botrytis cinerea n methanol + pectate
-
?
pectin + n H2O substrate is Citrus pectin with a degree of methyl esterification (DM) of 30%, 65% and 90%, best substrate for enzyme AtPME is pectin DM 90% at pH 7.5 Arabidopsis thaliana n methanol + pectate
-
?
pectin + n H2O substrate is Citrus pectin with a degree of methylesterification (DM) of 30%, 65% and 90%, best substrate for enzyme BcPME is pectin DM 90% at pH 6.0 Botrytis cinerea n methanol + pectate
-
?
pectin + n H2O substrate is Citrus pectin with a degree of methylesterification (DM) of 30%, 65% and 90%, best substrate for enzyme CsPME is pectin DM 90% at pH 7.5 Citrus sinensis n methanol + pectate
-
?
pectin + n H2O
-
Botrytis cinerea B05.10 n methanol + pectate
-
?
pectin + n H2O substrate is Citrus pectin with a degree of methylesterification (DM) of 30%, 65% and 90%, best substrate for enzyme BcPME is pectin DM 90% at pH 6.0 Botrytis cinerea B05.10 n methanol + pectate
-
?

Subunits

Subunits Comment Organism
? x * 32000, SDS-PAGE Citrus sinensis
? x * 40000, recombinant His-tagged enzyme, SDS-PAGE Arabidopsis thaliana
? x * 37000, recombinant enzyme, SDS-PAGE Botrytis cinerea

Synonyms

Synonyms Comment Organism
BcPME1
-
Botrytis cinerea
CsPME
-
Citrus sinensis
pectin methylesterase
-
Citrus sinensis
pectin methylesterase
-
Arabidopsis thaliana
pectin methylesterase
-
Botrytis cinerea
pectinesterase 31
-
Arabidopsis thaliana
PME
-
Citrus sinensis
PME
-
Arabidopsis thaliana
PME
-
Botrytis cinerea
PME31
-
Arabidopsis thaliana

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Citrus sinensis
30
-
assay at Arabidopsis thaliana
30
-
assay at Botrytis cinerea

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
85
-
purified enzyme, 10 min, inactivation Citrus sinensis
85
-
purified enzyme, 10 min, inactivation Arabidopsis thaliana
85
-
purified enzyme, 10 min, inactivation Botrytis cinerea

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4 6 compared to pH 7.5, homogalacturonans as substrates Botrytis cinerea
6 7.5 compared to pH 4.0, homogalacturonans as substrates Citrus sinensis
6 7.5 compared to pH 4.0, homogalacturonans as substrates Arabidopsis thaliana
6
-
compared to pH 4.0 and pH 7.5, Citrus pectins as substrates Botrytis cinerea
7.5
-
compared to pH 4.0 and pH 6.0, very low activity at pH 4.0, Citrus pectins as substrates Arabidopsis thaliana
7.5
-
compared to pH 6.0 and pH 4.0, low activity at pH 4.0, Citrus pectins as substrates Citrus sinensis

General Information

General Information Comment Organism
evolution sequence comparisons of pectinesterase enzymes from Citrus sinensis, Arabidopsis thaliana, and Botrytis cinerea Citrus sinensis
evolution sequence comparisons of pectinesterase enzymes from Citrus sinensis, Arabidopsis thaliana, and Botrytis cinerea Arabidopsis thaliana
evolution sequence comparisons of pectinesterase enzymes from Citrus sinensis, Arabidopsis thaliana, and Botrytis cinerea Botrytis cinerea
physiological function pectin methylesterases (PMEs) are present in phytopathogens such as bacteria and fungi Botrytis cinerea
physiological function pectin methylesterases (PMEs) play a central role in pectin remodeling during plant development Citrus sinensis
physiological function pectin methylesterases (PMEs) play a central role in pectin remodeling during plant development Arabidopsis thaliana