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Literature summary for 3.1.1.13 extracted from
- Hydrolysis of cholesteryl oleate liquid crystals by hormon-sensitive lipase (1993), J. Biochem., 113, 264-269.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| cAMP-dependent protein kinase type II | increasing activity on triolein in presence of Mg-ATP2- | Bos taurus | |
| phosphatidylcholine | slightly activating | Bos taurus |  |
| phosphatidylethanolamine | slightly activating | Bos taurus | |
General Stability
| General Stability | Organism |
|---|---|
| less stable in Tris-HCl buffer than in potassium phosphate buffer | Bos taurus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| phosphatidic acid | slight inhibition | Bos taurus | |
| phosphatidylcholine | slightly inhibitory at higher concentrations | Bos taurus | |
| phosphatidylethanolamine | - |
Bos taurus | |
| phosphatidylserine | complete inhibition at 0.05 mM for cholesteryl oleate hydrolysis, for triolein hydrolysis 65% inhibition, p-nitrophenyl butyrate hydrolysis not affected | Bos taurus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.011 | - |
cholesteryl oleate | in cholesteryl oleate vesicles | Bos taurus | |
| 0.012 | - |
cholesteryl oleate | in mixed emulsion of cholesteryl oleate, phosphatidylcholine and taurocholic acid | Bos taurus | |
| 0.099 | - |
cholesteryl oleate | in liquid crystals | Bos taurus | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| cholesteryl esters + H2O | Bos taurus | supplies free cholesterol as a precursor for steroidogenesis in steroid-producing tissues | cholesterol + fatty acid | - |
? | |
| cholesteryl esters + H2O | Bos taurus | involvement in arteriosclerosis | cholesterol + fatty acid | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bos taurus | - |
- |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| adipose tissue | - |
Bos taurus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.153 | - |
in liquid crystals of cholesteryl oleate | Bos taurus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| cholesteryl esters + H2O | supplies free cholesterol as a precursor for steroidogenesis in steroid-producing tissues | Bos taurus | cholesterol + fatty acid | - |
? | |
| cholesteryl esters + H2O | involvement in arteriosclerosis | Bos taurus | cholesterol + fatty acid | - |
? | |
| cholesteryl oleate + H2O | - |
Bos taurus | cholesterol + oleic acid | - |
? | |
| p-nitrophenyl butyrate + H2O | - |
Bos taurus | p-nitrophenol + butanoic acid | - |
? | |
| triolein + H2O | - |
Bos taurus | dioleoylglycerol + oleic acid | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| hormone-sensitive lipase | - |
Bos taurus |
| HSL | - |
Bos taurus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.8 | - |
measured in liquid crystals of cholesteryl oleate | Bos taurus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| MgATP2- | increasing activity with addition of cAMP-dependend protein kinase | Bos taurus | |
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Release 2023.1 (January 2023)
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