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Literature summary for 3.1.1.13 extracted from

  • DiPersio, L.P.; Kissel, J.A.; Hui, D.Y.
    Purification of pancreatic cholesterol esterase expressed in recombinant baculovirus-infected Sf9 cells (1992), Protein Expr. Purif., 3, 114-120.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
sodium taurocholic acid
-
Rattus sp.

Cloned(Commentary)

Cloned (Comment) Organism
overexpression in baculovirus-infected Sf9 cells Rattus sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
64000
-
gel filtration, SDS-PAGE, recombinant protein after 5-day incubation period Rattus sp.
74000
-
SDS-PAGE, recombinant protein Rattus sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
cholesteryl esters + H2O Rattus sp. mediating cholesterol absorption through the gastrointestinal tract, digestion and absorption of dietary fats cholesterol + fatty acid
-
?
cholesteryl esters + H2O Rattus sp. important role in fat digestion in infants cholesterol + fatty acid
-
?

Organism

Organism UniProt Comment Textmining
Rattus sp.
-
-
-

Purification (Commentary)

Purification (Comment) Organism
overexpression in Sf9 cells, heparin-Sepharose chromatography, gel filtration Rattus sp.

Source Tissue

Source Tissue Comment Organism Textmining
pancreas
-
Rattus sp.
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cholesteryl esters + H2O mediating cholesterol absorption through the gastrointestinal tract, digestion and absorption of dietary fats Rattus sp. cholesterol + fatty acid
-
?
cholesteryl esters + H2O important role in fat digestion in infants Rattus sp. cholesterol + fatty acid
-
?
p-nitrophenyl butyrate + H2O
-
Rattus sp. p-nitrophenol + butanoic acid
-
?

Subunits

Subunits Comment Organism
monomer 1 * 74000, SDS-PAGE Rattus sp.
monomer 1 * 64000, gel filtration, SDS-PAGE Rattus sp.