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Literature summary for 3.1.1.13 extracted from

  • Negre, A.; Salvayre, R.; Rogalle, P.; Dang, Q.Q.; Douste-Blazy, L.
    Acyl-chain specificity and properties of cholesterol esterases from normal and Wolman lymphoid cell lines (1987), Biochim. Biophys. Acta, 918, 76-82.
    View publication on PubMed
Search for more literature for 3.1.1.13

Activating Compound

Activating Compound Comment Organism Structure
sodium taurocholic acid optimal concentration for acid cholesteryl esterase at 2.5-5.0 mM/l, for neutral cholesteryl esterase at 0.1-1.0 mM/l Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
dithiothreitol for neutral cholesterol esterase with pH optimum at 6.0 and for acid cholesterol esterase Homo sapiens
E600 strong for neutral cholesterol esterases Homo sapiens
EDTA inhibition only for neutral cholesterol esterases Homo sapiens
Hg2+ strong for acid and less for neutral cholesterol esterases Homo sapiens
iodoacetate acid and neutral cholesterol esterases Homo sapiens
N-ethylmaleimide for acid and neutral cholesterol esterases Homo sapiens
NaCl acid and neutral cholesterol esterases Homo sapiens
p-hydroxymercuribenzoate strongest inactivation for acid cholesterol esterase, modest for neutral cholesterol esterase with pH optimum 6.0 Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ activation for neutral cholesterol esterases Homo sapiens
Mg2+ activation for neutral cholesterol esterases Homo sapiens
Mn2+ activation for neutral cholesterol esterases Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
cholesteryl esters + H2O Homo sapiens acid cholesterol esterase hydrolized only long-chain cholesteryl esters, activity is low for substrate concentration below the critical micelle concentration cholesterol + fatty acid
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
lymphoid tissue
-
 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cholesteryl acetate + H2O very slowly hydrolyzed by acid cholesterol esterase, only hydrolyzed by neutral cholestrol esterase at pH 8.0 Homo sapiens cholesterol + acetic acid
-
 ?
cholesteryl butyrate + H2O very slowly hydrolyzed by acid cholesterol esterase, only hydrolyzed by neutral cholesterol esterase at pH 8.0 Homo sapiens cholesterol + butanoic acid
-
 ?
cholesteryl caprylate + H2O
-
 Homo sapiens cholesterol + caprylic acid
-
 ?
cholesteryl esters + H2O acid cholesterol esterase hydrolized only long-chain cholesteryl esters, activity is low for substrate concentration below the critical micelle concentration Homo sapiens cholesterol + fatty acid
-
 ?
cholesteryl laurate + H2O
-
 Homo sapiens cholesterol + lauric acid
-
 ?
cholesteryl oleate + H2O
-
 Homo sapiens cholesterol + oleic acid
-
 ?

Synonyms

Synonyms Comment Organism
acid cholesterol esterase
-
 Homo sapiens
neutral cholesterol esterase
-
 Homo sapiens

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
55
-
 discriminated at acid pH, neutral cholesterol esterases more heat-labile than acid cholesterol esterase Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4
-
 for acid cholesterol esterase Homo sapiens
6
-
 for neutral cholesterol esterase I Homo sapiens
8
-
 for neutral cholesterol esterase II Homo sapiens