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Literature summary for 3.1.1.13 extracted from

  • Hui, D.Y.; Hayakawa, K.; Oizum, J.
    Lipoamidase activity in normal and mutagenized pancreatic cholesterol esterase (bile salt-stimulated lipase) (1993), Biochem. J., 291, 65-69.
No PubMed abstract available
Search for more literature for 3.1.1.13

Activating Compound

Activating Compound Comment Organism Structure
sodium taurocholic acid
-
 Homo sapiens
sodium taurocholic acid
-
 Rattus sp.

Cloned(Commentary)

Cloned (Comment) Organism
overexpression in baculovirus-infected Sf9 cells Rattus sp.

Protein Variants

Protein Variants Comment Organism
H435Q cholesterol esterase activity abolished , lipoamidase activity uneffected Rattus sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.011
-
 lipoyl 4-aminobenzoate mutant cholesterol esterase Rattus sp.
0.015
-
 Triacetin native cholesterol esterase Rattus sp.
0.024
-
 lipoyl 4-aminobenzoate native cholesterol esterase Rattus sp.

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Homo sapiens
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
72000
-
 calculation from sequence of cDNA Rattus sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
cholesteryl esters + H2O Homo sapiens shows also lipoamidase activity cholesterol + fatty acid
-
 ?
cholesteryl esters + H2O Rattus sp. shows also lipoamidase activity cholesterol + fatty acid
-
 ?
cholesteryl esters + H2O Homo sapiens important role in fat digestion in infants cholesterol + fatty acid
-
 ?
cholesteryl esters + H2O Rattus sp. important role in fat digestion in infants cholesterol + fatty acid
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-
Rattus sp.
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
milk
-
 Homo sapiens
-
pancreas
-
 Rattus sp.
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.027
-
 in absence of taurocholic acid Homo sapiens
0.045
-
 in presence of 2 mM taurocholic acid Homo sapiens
0.066
-
 in presence of 10 mM taurocholic acid Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cholesteryl esters + H2O shows also lipoamidase activity Homo sapiens cholesterol + fatty acid
-
 ?
cholesteryl esters + H2O shows also lipoamidase activity Rattus sp. cholesterol + fatty acid
-
 ?
cholesteryl esters + H2O important role in fat digestion in infants Homo sapiens cholesterol + fatty acid
-
 ?
cholesteryl esters + H2O important role in fat digestion in infants Rattus sp. cholesterol + fatty acid
-
 ?
lipoyl 4-aminobenzoate + H2O
-
 Homo sapiens 4-aminobenzoate + lipoic acid
-
 ?
lipoyl 4-aminobenzoate + H2O
-
 Rattus sp. 4-aminobenzoate + lipoic acid
-
 ?
p-nitrophenyl acetate + H2O
-
 Homo sapiens p-nitrophenol + acetic acid
-
 ?
p-nitrophenyl acetate + H2O
-
 Rattus sp. p-nitrophenol + acetic acid
-
 ?
triacetin + H2O
-
 Homo sapiens ? + acetic acid
-
 ?
triacetin + H2O
-
 Rattus sp. ? + acetic acid
-
 ?

Synonyms

Synonyms Comment Organism
bile salt-activated lipase
-
 Homo sapiens