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Literature summary for 3.1.1.13 extracted from

  • Pletnev, V.; Addlagatta, A.; Wawrzak, Z.; Duax, W.
    Three-dimensional structure of homodimeric cholesterol esterase-ligand complex at 1.4 A resolution (2003), Acta Crystallogr. Sect. D, 59, 50-56.
    View publication on PubMed
Search for more literature for 3.1.1.13

Crystallization (Commentary)

Crystallization (Comment) Organism
further purified commercial preparation enzyme bound to ligand of proposed formula C23H48O2, crystallization by hanging drop method from the monomeric fraction, 50 mM phosphate buffer, pH 7.3, containing PEG 3350, vapour diffusion against wells olution containing 21% PEG in the sam buffer, 293 K, 2 weeks, low-temperature X-ray diffraction structure determination and analysis at 1.4 A Limtongozyma cylindracea

Organism

Organism UniProt Comment Textmining
Limtongozyma cylindracea
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein 2 carbohydrate chains to Asn314 and Asn351, N-linked to N-acetyl-D-glucosamine moieties Limtongozyma cylindracea

Reaction

Reaction Comment Organism Reaction ID
steryl ester + H2O = sterol + fatty acid a group of enzymes of broad specificity, acting on esters of sterols and long-chain fatty acids, that may also bring about the esterification of sterols. Activated by bile salts, the catalytic tiad is formed by Ser209, Glu341, and His449 Limtongozyma cylindracea
a

Source Tissue

Source Tissue Comment Organism Textmining
commercial preparation
-
 Limtongozyma cylindracea
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cholesteryl acetate + H2O
-
 Limtongozyma cylindracea cholesterol + acetate
-
 ?
cholesteryl caprinate + H2O
-
 Limtongozyma cylindracea cholesterol + caprinate
-
 ?
cholesteryl caproate + H2O
-
 Limtongozyma cylindracea cholesterol + caproate
-
 ?
cholesteryl elaidate + H2O
-
 Limtongozyma cylindracea cholesterol + elaidate
-
 ?
cholesteryl linoleate + H2O best substrate Limtongozyma cylindracea cholesterol + linoleate
-
 ?
cholesteryl oleate + H2O
-
 Limtongozyma cylindracea cholesterol + oleate
-
 ?
cholesteryl palmitate + H2O
-
 Limtongozyma cylindracea cholesterol + palmitate
-
 ?
cholesteryl propionate + H2O
-
 Limtongozyma cylindracea cholesterol + propionate
-
 ?
cholesteryl stearate + H2O
-
 Limtongozyma cylindracea cholesterol + stearate
-
 ?
additional information enzyme prefers substrates of long-chain fatty acids and unsaturated fatty acids Limtongozyma cylindracea ?
-
 ?

Subunits

Subunits Comment Organism
dimer crystal structure Limtongozyma cylindracea
More dimer with four spatially separated interfacial contact areas and two symmetry-related pairs of openings to an internal intradimer cavity Limtongozyma cylindracea

Synonyms

Synonyms Comment Organism
ChE
-
 Limtongozyma cylindracea
cholesterol esterase
-
 Limtongozyma cylindracea