Activating Compound | Comment | Organism | Structure |
---|---|---|---|
cholate | up to 100 mM, enhancement of the activity, bile salt is absolutely required for activity | Pseudomonas aeruginosa | |
taurocholate | up to 100 mM, enhancement of the activity, bile salt is absolutely required for activity | Pseudomonas aeruginosa |
Application | Comment | Organism |
---|---|---|
additional information | in presence of taurocholate the enzyme is an effective cleaner for contact lenses stained with lipids consisting of cholesteryl oleate, tripalmitin, and stearyl stearate | Pseudomonas aeruginosa |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | no inhibition by iodoacetic acid, DTNB, EDTA and tetranitromethane | Pseudomonas aeruginosa | |
PMSF | - |
Pseudomonas aeruginosa | |
Triton X-100 | - |
Pseudomonas aeruginosa |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | divalent metal ions do not influence the enzyme activity | Pseudomonas aeruginosa |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
53000 | - |
gel filtration | Pseudomonas aeruginosa |
58000 | - |
x * 58000, SDS-PAGE | Pseudomonas aeruginosa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas aeruginosa | - |
- |
- |
Purification (Comment) | Organism |
---|---|
116fold | Pseudomonas aeruginosa |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
8.5 | - |
purified enzyme | Pseudomonas aeruginosa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
cholesteryl acetate + H2O | low activity, 6% of the activity with cholesteryl linoleate | Pseudomonas aeruginosa | cholesterol + acetate | - |
? | |
cholesteryl butyrate + H2O | low activity, 8% of the activity with cholesteryl linoleate | Pseudomonas aeruginosa | cholesterol + butyrate | - |
? | |
cholesteryl caprate + H2O | 40% of the activity with cholesteryl linoleate | Pseudomonas aeruginosa | cholesterol + caprate | - |
? | |
cholesteryl caproate + H2O | 22% of the activity with cholesteryl linoleate | Pseudomonas aeruginosa | cholesterol + caproate | - |
? | |
cholesteryl caprylate + H2O | 62% of the activity with cholesteryl linoleate | Pseudomonas aeruginosa | cholesterol + caprylate | - |
? | |
cholesteryl laurate + H2O | 41% of the activity with cholesteryl linoleate | Pseudomonas aeruginosa | cholesterol + laurate | - |
? | |
cholesteryl linoleate + H2O | best substrate | Pseudomonas aeruginosa | cholesterol + linoleate | - |
? | |
cholesteryl myristate + H2O | 58% of the activity with cholesteryl linoleate | Pseudomonas aeruginosa | cholesterol + myristate | - |
? | |
cholesteryl oleate + H2O | 92% of the activity with cholesteryl linoleate | Pseudomonas aeruginosa | cholesterol + oleate | - |
? | |
cholesteryl palmitate + H2O | 75% of the activity with cholesteryl linoleate | Pseudomonas aeruginosa | cholesterol + palmitate | - |
? | |
cholesteryl stearate + H2O | 80% of the activity with cholesteryl linoleate | Pseudomonas aeruginosa | cholesterol + stearate | - |
? | |
triolein + H2O | positional specificity for sn-2-position, lower affinity for the sn-1- and sn-3-position | Pseudomonas aeruginosa | diolein + oleate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 58000, SDS-PAGE | Pseudomonas aeruginosa |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
53 | - |
- |
Pseudomonas aeruginosa |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
53 | - |
30 min, pH 7.0, stable | Pseudomonas aeruginosa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Pseudomonas aeruginosa |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.5 | 9.5 | - |
Pseudomonas aeruginosa |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Pseudomonas aeruginosa | isoelectric focusing | - |
3.2 |