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Literature summary for 3.1.1.14 extracted from
- Purification and properties of chlorophyllase from greened rye seedlings (1982), J. Biochem., 92, 1763-1773.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| acetone | 30% acetone stimulates hydrolysis of bacteriochlorophyll by 50%, stimulates hydrolysis of chlorophyll a by about 400% | Secale cereale |  |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| PCMB | - |
Secale cereale | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.004 | - |
bacteriochlorophyll a | pH 7.5, 30°C | Secale cereale | |
| 0.012 | - |
chlorophyll a | pH 7.5, 30°C | Secale cereale | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 39000 | - |
gel filtration | Secale cereale |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Secale cereale | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Secale cereale |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| seedling | - |
Secale cereale | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.48 | - |
- |
Secale cereale |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| bacteriochlorophyll a + H2O | - |
Secale cereale | bacteriochlorophyllide a + phytol | - |
? | |
| chlorophyll a + H2O | - |
Secale cereale | chlorophyllide a + phytol | - |
? | |
| chlorophyll b + H2O | - |
Secale cereale | chlorophyllide b + phytol | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
- |
Secale cereale |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
not stable above a 10% concentration of acetone | Secale cereale |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 7.5 | hydrolysis of bacteriochlorophyll a | Secale cereale |
| 7 | 8.5 | hydrolysis of chlorophyll a | Secale cereale |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 9 | 4°C, 24 h, stable | Secale cereale |
| 7.5 | - |
stable in presence or absence of 30% acetone | Secale cereale |
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