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Literature summary for 3.1.1.2 extracted from
- Membrane marker enzymes. Characterization of an arylesterase of guinea pig cerebral cortex utilizing p-nitrophenyl acetate as substrate (1972), Biochim. Biophys. Acta, 276, 180-191.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | - |
Cavia porcellus |  |
| Co2+ | - |
Cavia porcellus | |
| Cu2+ | - |
Cavia porcellus | |
| Fe2+ | - |
Cavia porcellus | |
| Hg2+ | - |
Cavia porcellus | |
| Mg2+ | not | Cavia porcellus | |
| additional information | not: Cd2+ | Cavia porcellus | |
| p-hydroxymercuribenzoate | - |
Cavia porcellus | |
| Pb2+ | - |
Cavia porcellus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.000043 | - |
4-nitrophenyl acetate | 37°C, pH 7.6 | Cavia porcellus | |
| 0.043 | - |
p-nitrophenyl acetate | - |
Cavia porcellus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| microsome | - |
Cavia porcellus | - |
- |
| microsome | good marker enzyme | Cavia porcellus | - |
- |
| rough endoplasmic reticulum | good marker enzyme | Cavia porcellus | 5791 | - |
| synaptosome | - |
Cavia porcellus | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Cd2+ | slightly activating | Cavia porcellus | |
| Cd2+ | can partially replace Mn2+ in activation | Cavia porcellus | |
| Mg2+ | slightly acitvating | Cavia porcellus | |
| Mn2+ | MnCl2 required | Cavia porcellus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 78000 | - |
and 180000, whether the former is a subunit of the latter is not known, gel filtration | Cavia porcellus |
| 180000 | - |
and 78000, whether the latter is a subunit of the former is not known, gel filtration | Cavia porcellus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| phenyl acetate + H2O | Cavia porcellus | - |
? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Cavia porcellus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Cavia porcellus |
| 2 forms, pI 4.9 form and pI 5.9 form | Cavia porcellus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| cerebral cortex | - |
Cavia porcellus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 155 | - |
- |
Cavia porcellus |
| 155.6 | - |
37°C, pH 7.6 | Cavia porcellus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrophenyl acetate + H2O | - |
Cavia porcellus | 4-nitrophenol + acetate | - |
? | |
| 4-nitrophenyl acetate + H2O | - |
Cavia porcellus | 4-nitrophenol + acetate | - |
ir | |
| phenyl acetate + H2O | - |
Cavia porcellus | ? | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Cavia porcellus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 100 | - |
boiling completely abolishes activity | Cavia porcellus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.6 | - |
- |
Cavia porcellus |
| 7.6 | - |
p-nitrophenyl acetate | Cavia porcellus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 9 | p-nitrophenyl acetate | Cavia porcellus |
| 7.3 | 8.2 | about 50% of activity maximum at pH 7.3 and 8.2 | Cavia porcellus |
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