Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Nonidet P-40 | 8.2% activation at 5% (v/v) | uncultured organism | |
Triton X-100 | 11.2% activation at 5% (v/v) | uncultured organism | |
Tween 20 | 4.8% activation at 5% (v/v) | uncultured organism | |
Tween 40 | 16.4% activation at 5% (v/v) | uncultured organism | |
Tween 80 | 19.6% activation at 5% (v/v) | uncultured organism |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | uncultured organism |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | 73.5% residual activity at 5 mM | uncultured organism | |
Ag+ | 9.7% residual activity at 5 mM | uncultured organism | |
Ca2+ | 88.5% residual activity at 5 mM | uncultured organism | |
Co2+ | 92.6% residual activity at 5 mM | uncultured organism | |
Cr3+ | 39.8% residual activity at 5 mM | uncultured organism | |
Cu2+ | 28.9% residual activity at 5 mM | uncultured organism | |
diethyl dicarbonate | 5.5% residual activity at 5 mM | uncultured organism | |
dithiothreitol | 19.4% residual activity at 5 mM | uncultured organism | |
EDTA | 94.2% residual activity at 5 mM | uncultured organism | |
Fe3+ | 13.7% residual activity at 5 mM | uncultured organism | |
Hg2+ | 7.2% residual activity at 5 mM | uncultured organism | |
Mg2+ | 89.2% residual activity at 5 mM | uncultured organism | |
Mn2+ | 85.4% residual activity at 5 mM | uncultured organism | |
additional information | addition of glycerin has no effect on the enzyme activity | uncultured organism | |
NaCl | 43.7% residual activity at 4 M | uncultured organism | |
Ni2+ | 86.3% residual activity at 5 mM | uncultured organism | |
Pb2+ | 45.7% residual activity at 5 mM | uncultured organism | |
phenylmethylsulfonyl fluoride | complete inhibition at 5 mM | uncultured organism | |
SDS | complete inhibition at 5% (w/v) | uncultured organism | |
Urea | 64.7% residual activity at 8 M | uncultured organism | |
Zn2+ | 76.4% residual activity at 5 mM | uncultured organism |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.041 | - |
1-naphthyl acetate | in 50 mM Tris-HCl (pH 8.0), at 37°C | uncultured organism | |
0.53 | - |
phenyl acetate | in 50 mM Tris-HCl (pH 8.0), at 37°C | uncultured organism | |
0.96 | - |
4-nitrophenyl acetate | in 50 mM Tris-HCl (pH 8.0), at 37°C | uncultured organism |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
20000 | - |
x * 20000, SDS-PAGE | uncultured organism |
24000 | - |
x * 24000, calculated from amino acid sequence | uncultured organism |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
uncultured organism | G0YFD9 | - |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation, Q Sepharose column chromatography, and Superdex 75 gel filtration | uncultured organism |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | bacterium from goat rumen content | uncultured organism | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-naphthyl acetate + H2O | 15.4% activity compared to 4-nitrophenyl acetate | uncultured organism | 1-naphthol + acetate | - |
? | |
4-nitrophenyl acetate + H2O | highest catalytic efficiency (100% activity) | uncultured organism | 4-nitrophenol + acetate | - |
? | |
4-nitrophenyl butyrate + H2O | 19.7% activity compared to 4-nitrophenyl acetate | uncultured organism | 4-nitrophenol + butyrate | - |
? | |
4-nitrophenyl caproate + H2O | 4.1% activity compared to 4-nitrophenyl acetate | uncultured organism | 4-nitrophenol + caproate | - |
? | |
additional information | the enzyme has no activity towards 4-nitrophenyl esters with acyl chains longer than C8 like 4-nitrophenyl caprylate, 4-nitrophenyl caprate, 4-nitrophenyl palmitate, as well as 4-nitrophenyl phosphate and methyl parathion | uncultured organism | ? | - |
? | |
phenyl acetate + H2O | 166% activity compared to 4-nitrophenyl acetate | uncultured organism | phenol + acetate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 20000, SDS-PAGE | uncultured organism |
? | x * 24000, calculated from amino acid sequence | uncultured organism |
Synonyms | Comment | Organism |
---|---|---|
EstR5 | - |
uncultured organism |
SGNH arylesterase | - |
uncultured organism |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
- |
uncultured organism |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | 65 | the enzyme exhibits more than 50% of the maximal activity between 40°C and 65°C | uncultured organism |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | 60 | the enzyme is stable at 45°C, retaining 91.5% of the activity after 1 h incubation. At 60°C, the enzyme activity decreases rapidly and only retains 16.8% of the activity after 1 h incubation | uncultured organism |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
uncultured organism |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 9 | more than 60% of the maximum activity is retained between pH 6.0 and 9.0 | uncultured organism |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5 | 11 | the enzyme is stable between pH 5.0 and 11.0, retaining more than 50% of the initial activity after incubation at 37°C for 1 h | uncultured organism |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
uncultured organism | calculated from amino acid sequence | - |
4.6 |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
204.5 | - |
4-nitrophenyl acetate | in 50 mM Tris-HCl (pH 8.0), at 37°C | uncultured organism | |
401.4 | - |
phenyl acetate | in 50 mM Tris-HCl (pH 8.0), at 37°C | uncultured organism | |
1612 | - |
1-naphthyl acetate | in 50 mM Tris-HCl (pH 8.0), at 37°C | uncultured organism |